Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-GEOD-22434

ABSTRACT: Expression data from Evi1-transduced primary bone marrow cells

OTHER RELATED OMICS DATASETS IN: PRJNA128547

PROVIDER: E-GEOD-22434 | ExpressionAtlas | 2015-09-15

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-GEOD-22434-analysis-methods.tsv	Tabular
	E-GEOD-22434-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-22434-configuration.xml	Xml
	E-GEOD-22434-percentile-ranks.tsv	Tabular
	E-GEOD-22434.condensed-sdrf.tsv	Tabular

Items per page:

1 - 5 of 47

Similar Datasets

E-GEOD-53916

Project description:Expression data from mouse bone marrow cells expressing renin driven expression of green fluorescent protein.

2015-07-31 | E-GEOD-53916 | ExpressionAtlas

E-GEOD-63653

Project description:Expression data from bone marrow-derived macrophages

2016-05-03 | E-GEOD-63653 | ExpressionAtlas

Mus musculus

Project description:Expression data from Evi1-transduced primary bone marrow cells

| PRJNA128547 | ENA

E-GEOD-54073

Project description:Gene expression microarray data of bone marrow derived macrophages using GM-CSF(GM-BMMs) co-cultured with E0771 breast tumor cells

2014-09-08 | E-GEOD-54073 | ExpressionAtlas

E-GEOD-42257

Project description:Murine bone marrow gene expression profiling

2015-08-06 | E-GEOD-42257 | ExpressionAtlas

E-GEOD-21905

Project description:Gene expression in Lineage negative bone marrow cells of C57B/L6 and B.D(Chr5) mice

2015-09-15 | E-GEOD-21905 | ExpressionAtlas

Expression data from Evi1-transduced primary bone marrow cells

Project description:Evi1 is essential for proliferation of hematopoietic stem cells and implicated in the development of myeloid disorders. Particularly, high Evi1 expression defines one of the largest clusters in acute myeloid leukemia and is significantly associated with extremely poor prognosis. Improvement of the therapeutic outcome of leukemia with activated Evi1 is one of the most challenging issues. However, mechanistic basis of Evi1-mediated leukemogenesis has not been fully elucidated. Here we show that Evi1 directly represses PTEN transcription in the murine bone marrow, which leads to activation of AKT/mTOR signaling. In a murine bone marrow transplantation model, Evi1 leukemia showed remarkable sensitivity to an mTOR inihibitor rapamycin. Furthermore, we found that Evi1 binds to several polycomb group proteins and recruits polycomb repressive complexes for PTEN downregulation, which reveals a novel epigenetic mechanism of AKT/mTOR activation in leukemia. Expression analyses and chromatin immunoprecipitation assays using human samples indicate that our findings in mice models are recapitulated in human leukemic cells. Dependence of Evi1-expressing leukemic cells on AKT/mTOR signaling provides the first example of targeted therapeutic modalities that suppress the leukemogenic activity of Evi1. The PTEN/AKT/mTOR signaling pathway and the Evi1-polycomb interaction can be promising therapeutic targets for leukemia with activated Evi1. Gene expression analysis for the purpose of identifying the target genes of Evi1 in primary bone marrow. 5-FU-primed mononuclear bone marrow cells harvested from C57/B6 mice were retrovirally transduced with Evi1-GFP or GFP. GFP positive cells were sorted and analyzed by AffymetrixÂ® Mouse Genome 430 2.0 ArrayÂ® for gene expression. Four independent experiments were performed.

2010-07-08 | E-GEOD-22434 | biostudies-arrayexpress

E-GEOD-1571

Project description:Gene expression of side population and main population cells, isolated from adult murine skeletal muscle and bone marrow

2015-09-03 | E-GEOD-1571 | ExpressionAtlas

E-GEOD-43806

Project description:Klf5 controls bone marrow homing of stem cells and progenitors through Rab5-mediated membrane Beta1/Beta2-integrin expression

2015-07-10 | E-GEOD-43806 | ExpressionAtlas

E-GEOD-35825

Project description:Type I and II Interferon-Stimulated Genes in Murine Primary Bone Marrow Macrophages

2015-09-28 | E-GEOD-35825 | ExpressionAtlas