Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-GEOD-22772

ABSTRACT: Expression profile of U373 cells over-expressing DTX1 and cells expressing a dominant negative form of MAML1

OTHER RELATED OMICS DATASETS IN: PRJNA128263

PROVIDER: E-GEOD-22772 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-GEOD-22772-analysis-methods.tsv	Tabular
	E-GEOD-22772-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-22772-configuration.xml	Xml
	E-GEOD-22772-percentile-ranks.tsv	Tabular
	E-GEOD-22772.condensed-sdrf.tsv	Tabular

Items per page:

1 - 5 of 71

Similar Datasets

E-GEOD-38007

Project description:Gene expression profile of MITF expressing cells

2025-01-24 | E-GEOD-38007 | ExpressionAtlas

Expression data from U373 cells expressing EGFP, MAML1-dn and DTX1-myc

Project description:Glioblastoma multiforme (GBM) is the most malignant and most common tumor of the central nervous system characterized by rapid growth and extensive tissue infiltration. GBM results in more years of life lost than any other cancer type. Notch signaling has been implicated in GBM pathogenesis through several modes of action. Inhibition of Notch leads to a reduction of cancer-initiating cells in gliomas and reduces proliferation and migration. Deltex1 (DTX1) is part of an alternative Notch signaling pathway distinct from the canonical MAML1/RBPJŒ∫-mediated cascade. In this study, we show that DTX1 activates both the RTK/PI3K/PKB as well as the MAPK/ERK pathway. Moreover, we found the anti-apoptotic factor Mcl-1 to be induced by DTX1. In accordance with this, the clonogenic potential and proliferation rates of glioma cell lines correlated with DTX1 levels. DTX1 knock down mitigated the tumorigenic potential in vivo, and overexpression of DTX1 increased cell migration and invasion of tumor cells accompanied by an elevation of the pro-migratory factors PKBŒ≤ and Snail1. Microarray gene expression analysis identified a DTX1-specific transcriptional program - including microRNA-21 - which is distinct from the canonical Notch signaling. We propose the alternative Notch pathway via DTX1 as oncogenic factor in malignant glioma and found low DTX1 expression levels to correlate with prolonged survival of GBM and early breast cancer patients in open source databases. We generated human glioma U373 cell lines stably expressing Enhanced Green Fluorescent Protein (EGFP), human Deltex1-Myc Tag (DTX1-myc), or Master Mind Like 1 - dominant negative (MAML1-dn) to compare differences in overall gene expression. We included 3x EGFP control samples, 3x DTX1-myc, and 3 MAML1-dn samples.

2012-05-31 | E-GEOD-22772 | biostudies-arrayexpress

E-MEXP-1274

Project description:Transcription profiling of human bronchial epithelial cells stabily expressing a dominant negative form of TLR3 after infection with influenza A virus or not infected

2025-01-22 | E-MEXP-1274 | ExpressionAtlas

Next Generation Sequencing of human trophoblast stem cells expressing a doxycycline-inducible dominant-negative MAML1 construct.

Project description:Mastermind-like 1 (MAML1) functions as a co-activator in the NOTCH signaling pathway. A truncated version of its wild type form, the so-called dominant-negative MAML1 (DN-MAML1) , lacks the transactivation domains that are needed for stimulation of gene expression. The aim of the study was to characterize the effects of NOTCH signaling pathway inhibition in human trophoblast stem cells (TSCs) by overexpression of DN-MAML1.

2023-06-30 | GSE233140 | GEO

E-GEOD-35395

Project description:Gene expression profile of c-kit positive and c-kit negative cells

2015-09-22 | E-GEOD-35395 | ExpressionAtlas

Homo sapiens

Project description:Expression data from U373 cells expressing EGFP, MAML1-dn and DTX1-myc

| PRJNA128263 | ENA

E-GEOD-15811

Project description:Gene expression profile of human cord blood CD34+ cells expressing ZMYM2/FGFR1, BCR/FGFR1 or BCR/ABL1

2025-01-24 | E-GEOD-15811 | ExpressionAtlas

E-MTAB-2287

Project description:Gene expression profile in wheat TaRZ1-expressing Arabidopsis plant

2015-06-10 | E-MTAB-2287 | ExpressionAtlas

E-GEOD-22385

Project description:Gene profiling: U87 IRE1 dominant negative cells vs. U87ctrl cells in culture

2025-01-24 | E-GEOD-22385 | ExpressionAtlas

E-GEOD-2192

Project description:Transcription profiling by array of mouse NIH3T3 cells over-expressing PPARg pr EBF1 over time

2014-08-29 | E-GEOD-2192 | ExpressionAtlas

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data