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E-GEOD-26259

ABSTRACT: Gene expression profiles of MCF7 with CARM1 knocked down

OTHER RELATED OMICS DATASETS IN: PRJNA135097PRJNA136557

PROVIDER: E-GEOD-26259 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

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Dataset's files

Source:

			Action	DRS
	E-GEOD-26259-analysis-methods.tsv	Tabular
	E-GEOD-26259-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-26259-configuration.xml	Xml
	E-GEOD-26259-percentile-ranks.tsv	Tabular
	E-GEOD-26259.condensed-sdrf.tsv	Tabular

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Project description:The goal of this study is to identify ERalpha-target genes affected by knocking down of the histone arginine methyltransferase CARM1 in MCF7 breast cancer cells. The roles of CARM1 in ERalpha+ breast cancer was not well characterized. Therefore, we created a Dox inducible CARM1 knockingdown MCF7 cell line where CARM1 is decreased to 20% of endogeneous level to determine the created a Dox-inducible CARM1shRNA overexpressing MCF7 cells for evaluation of the global effects of CARM1 on ERalpha-target gene expression. MCF7-tet-on-CARM1shRNA clone 1 were treated under 4 conditions: DMSO; Dox; E2 (10nM); Dox+E2. In Dox+E2 condition, cells were pre-treated with Dox for 5 days before treating with E2 for 4 hours. 3 biological replicates were included and total of 12 samples were analyzed.

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