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E-GEOD-29814

ABSTRACT: Transcription profiling by array of Arabidopsis speechless, scrm-D and scrm-D;mute mutant seedlings

OTHER RELATED OMICS DATASETS IN: PRJNA140931

PROVIDER: E-GEOD-29814 | ExpressionAtlas | 2014-07-03

REPOSITORIES: ExpressionAtlas

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Dataset's files

Source:

			Action	DRS
	E-GEOD-29814-analysis-methods.tsv	Tabular
	E-GEOD-29814-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-29814-configuration.xml	Xml
	E-GEOD-29814-percentile-ranks.tsv	Tabular
	E-GEOD-29814-zoomifications-log.tsv	Tabular

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Molecular profiling of stomatal lineage cell states

Project description:We initiated a study to investigate the transcriptional profiles associated with cell states of the stomatal lineage. A stem-cell like precursor of stomata, a meristemoid. reiterates asymmetric divisions and renews itself before differentiating into guard cells. The transient and asynchronous nature of the meristemoid has made it difficult to study its molecular characteristics. Through combinatorial use of genetic resources that either arrest or constitutively drive stomatal cell-state progressions due to loss- or gain-of-function mutations in the key transcription factor genes, SPEECHLESS, MUTE, and SCRM, we obtained seedlings highly enriched in pavement cells, meristemoids, or stomata. Here we present transcriptome and genome-wide trends in gene regulation associated with each cell state and identify molecular signatures associated with meristemoids. 12 samples are included in this study. Three biological replicates of 5-dag seedlings of speechless, scrm-D and scrm-D;mute were compared wild type seedlings for changes in gene expression.

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