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E-GEOD-38430

ABSTRACT: Gene expression data comparing rbf1 mutant and PSC overexpressing Drosophila eye imaginal discs

PROVIDER: E-GEOD-38430 | ExpressionAtlas | 2015-07-08

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-38430-analysis-methods.tsv	Tabular
	E-GEOD-38430-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-38430-configuration.xml	Xml
	E-GEOD-38430-percentile-ranks.tsv	Tabular
	E-GEOD-38430.condensed-sdrf.tsv	Tabular

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Drosophila melanogaster

Project description:Gene expression data comparing rbf1 mutant and PSC overexpressing Drosophila eye imaginal discs

| PRJNA167839 | ENA

Gene expression data comparing rbf1 mutant and PSC overexpressing Drosophila eye imaginal discs

Project description:PSC overexpression can cause phenotypes specifically in an rbf1 mutant background, likely due to a sensitization to PSC-induced phenotypes. The goal of this study is to understand the interaction between rbf1 hypomorphic mutation and the overexpression of Polycomb group gene Posterior sex combs. We used Drosophila larval eye imaginal discs that were mutant for rbf1 or overexpressing PSC and compared these to control larval eye discs to assess changes in gene expression. We identified a common set of genes that are deregulated when rbf1 is mutated or when PSC is overexpressed. RNA was extracted from eye imaginal discs dissected from third instar Drosophila larvae. Samples were amplified and hybridized to Affymetrix Drosophila Genome 2.0 Array. To better understand the effects of rbf1 mutation and PSC overexpression, we compared the gene expression of rbf1 mutant eye discs and eye discs overexpressing PSC to control eye discs.

2014-09-30 | E-GEOD-38430 | biostudies-arrayexpress

Gene expression data comparing rbf1 mutant and PSC overexpressing Drosophila eye imaginal discs

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Analysis of H3K9ac in fly imaginal discs [ChIP-seq]

Project description:We have analyzed ChIP-Seq of H3K9ac and H3K27me3 in wing imaginal discs and eye imaginal discs from Drosophila melanogaster.

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Tag-seq in eye-antennal and wing imaginal discs of D.melanogaster, D.yakuba, and D.virilis; RNA-seq in wild-type and glass mutant D.melanogaster eye-antennal imaginal discs.

Project description:In this study we use Tag-sequencing in eye-antennal and wing imaginal discs across Drosophila species to determine a set of conserved eye-specific developmental genes. Next, we perform motif discovery analysis using the tool i-cisTarget, to depict the core gene developmental network underlying compound eye photoreceptor. The Glass position weight matrix appears as the most highly overrepresented motif, thus positioning Glass as a master regulator in compound eye photoreceptor development. Differential gene expression analysis by RNA-seq in D.melanogaster wild-type eye-antennal versus glass mutant [gl 60j] shows that the majority of our predicted Glass targets show strong downregulation in the glass mutant. This SuperSeries is composed of the following subset Series: GSE39781: RNA-seq in wild-type and glass mutant eye-antennal discs in Drosophila melanogaster GSE39782: Tag-seq profiling in eye-antennal and wing imaginal discs of D. melanogaster, D. yakuba and D. virilis

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Transcriptional activation without chromatin marking in developmentally regulated genes [ChIP-seq]

Project description:We have conducted ChIP-Seq of H3K4me1/H3K27ac in order to perform a comparative study between wing imaginal discs and eye imaginal discs from Drosophila melanogaster.

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Chromatin marking is associated to transcriptional stability during fly development

Project description:We have conducted ChIP-Seq and RNA-Seq analyses in order to perform a comparative study between wing imaginal discs and eye imaginal discs from Drosophila melanogaster.

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Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

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