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E-GEOD-40549

ABSTRACT: Spatial transcriptomes of iron-deficient and cadmium-stressed rice

OTHER RELATED OMICS DATASETS IN: PRJNA174253

PROVIDER: E-GEOD-40549 | ExpressionAtlas | 2015-12-04

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-40549-analysis-methods.tsv	Tabular
	E-GEOD-40549-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-40549-configuration.xml	Xml
	E-GEOD-40549-percentile-ranks.tsv	Tabular
	E-GEOD-40549.condensed-sdrf.tsv	Tabular

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Project description:Spatial transcriptomes of iron-deficient and cadmium-stressed rice

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Spatial transcriptomes of iron-deficient and cadmium-stressed rice

Project description:Several metals are essential nutrients for plants but metals are toxic in excess, deleteriously affecting crop yield and quality. Various kinds of genes involved in metal homeostasis have been investigated in detail over the past few decades and the mechanisms of how metals are absorbed from soil and distributed in plants have been elucidated. However, numerous genes related to metal homeostasis remain to be investigated and a comprehensive analysis of the expressions of these genes is required. In the present study, we investigated the spatial gene expression profile of iron (Fe)-deficient and cadmium (Cd)-stressed rice by a combination of laser capture microdissection and microarray analysis. We performed comprehensive microarray analysis of a rice root using laser microdissection and collected a total of 13 samples (3 replicates for each sample, 39 total microarray data). Roots of normal, Fe-deficient (-Fe) and Cd-stressed (+Cd) rice were separated into the vascular bundle (VB), cortex (Cor), and epidermis plus exodermis (EP). In addition, vascular bundles from new leaves (newDC) and old leaves (oldDC) at the lowest node, which are important for metal distribution, were separately analyzed.

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Characterizing the role of rice NRAMP5 in Manganese, Iron and Cadmium Transport

Project description:OsNRAMP5 is involved in iron, manganese and cadmium transport. Goal was to determine the effects of knocking down OsNRAMP5 on global rice gene expression. Two experiments: Control condition experiment: root or shoot of WT vs. OsNRAMP5 RNAi plants. WT Cd treatment experiment: 4 weeks old WT plants grown under 0 or 10uM Cd for two weeks

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Project description:Transcription profiling by array of Arabidopsis Kas-1 and Tsu-1 grown in iron-deficient medium

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