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E-GEOD-46086

ABSTRACT: DNA microarray data from root of transgenic rice Huahui 1 (HH1) and its parent Minghui 63 (MH63)

PROVIDER: E-GEOD-46086 | ExpressionAtlas | 2015-11-20

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-46086-analysis-methods.tsv	Tabular
	E-GEOD-46086-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-46086-configuration.xml	Xml
	E-GEOD-46086-percentile-ranks.tsv	Tabular
	E-GEOD-46086.condensed-sdrf.tsv	Tabular

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DNA microarray data from root of transgenic rice Huahui 1 (HH1) and its parent Minghui 63 (MH63)

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DNA microarray data from root of transgenic rice Huahui 1 (HH1) and its parent Minghui 63 (MH63)

Project description:DNA microarray analysis has been proved to be an effective method in investigating unintended effects in genetically modified (GM) crops. However, unintended effects of GM plants in leaves through DNA microarray analysis has many researches, but research of unintended effects of GM plants of the underground portion has few. In this study, DNA microarray analysis was used to detect DEG in underground portions between transgenic rice HH1 and its non-transgenic control MH63. We used microarrays to study unintended effects in root of transgenic rice Huahui 1. Samples were collected from root of HH1 and MH63 at 30-day old and were used for RNA extraction and hybridization on Affymetrix microarrays. We selected diferentially expressed genes and common significantly changed pathways hoping to as a clue to investigate unintended effects of HH1 root.

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Project description:Two-week old bread wheat seedlings hydroponically grown Hoagland solution were transferred to potassium (K+)-free conditions for 8 d, their root and leaf proteome profiles were assessed using iTRAQ proteome method, and NCBInr database combined with the recently published bread wheat genome information were used to analyze the identified protein species. Over 4,000 unique proteins were identified, 818 K+-responsive protein species showed significant abundance regulation. The most majority of the identified K+-responsive protein species had exact gene loci, and showed no global but tissue- and chromosome- dependent genome distributions. The identified protein species were associated with diverse functions and exhibited organ-specific differences. Most of identified protein species associated with hormone synthesis were the enzymes involved in the synthesis of jasmonic acid (JA). Allene oxide synthase (AOS), a key JA synthesis-related enzyme, was significantly induced in both root and leaf organs of K+-deficient wheat seedlings, and its overexpression in rice enhanced the tolerance to low K+ or K+ deficiency, increased contents of K+ and JA and transcription levels of some K+-responsive genes. However, rice AOS T-DNA inserted mutant (osaos) exhibited more sensitivity to K+ deficiency. K+ deficiency significantly increased abundance of a high affinity K+ transporter (TaAHAK1), TaAHAK1 transgenic rice seedlings markedly alleviated sensitivity to K+ deficiency, and K+ deficiency also upregulated expression of homologous OsHAK1 gene in TaAOS transgenic rice plants. These results suggested an essential role of JA in K+ deficiency and gave molecular insight into the responses of plant to K+ deficiency.

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Project description:DNA microarray data from transgenic rice Huahui 1 (HH1) and its parent Minghui 63 (MH63)

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