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E-GEOD-53604

ABSTRACT: Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPaseold-35)

OTHER RELATED OMICS DATASETS IN: PRJNA232478

PROVIDER: E-GEOD-53604 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-GEOD-53604-analysis-methods.tsv	Tabular
	E-GEOD-53604-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-53604-configuration.xml	Xml
	E-GEOD-53604-percentile-ranks.tsv	Tabular
	E-GEOD-53604.condensed-sdrf.tsv	Tabular

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Project description:Human Polynucleotide Phosphorylase (hPNPaseold-35) is an evolutionarily conserved 3’→5’ exoribonuclease implicated in the regulation of numerous physiological processes like maintenance of mitochondrial homeostasis, mtRNA import and aging-associated inflammation. From an RNase perspective, little is known about the RNA or miRNA species it targets for degradation or whose expression it regulates; except for c-myc and miR-221. To further elucidate the functional implications of hPNPaseold-35 in cellular physiology, we knocked-down and overexpressed hPNPaseold-35 in melanoma cells and performed gene expression analyses to identify differentially expressed transcripts. Biological triplicates were run on microarrays.

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