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E-GEOD-68284

ABSTRACT: Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes at post natal day 21 [RNA-Seq P21]

OTHER RELATED OMICS DATASETS IN: PRJNA282464PRJNA282463PRJNA282462PRJNA266762

PROVIDER: E-GEOD-68284 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-68284-analysis-methods.tsv	Tabular
	E-GEOD-68284-analytics.tsv	Tabular
	E-GEOD-68284-analytics.tsv.undecorated	Tabular
	E-GEOD-68284-analytics.tsv.undecorated.unrounded	Tabular
	E-GEOD-68284-analytics.tsv.unrounded	Tabular

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E-GEOD-68283

Project description:Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes at post natal day 30 [RNA-Seq P30]

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Project description:Purpose: The goals of this study are to compare Next-generation sequencing (NGS)-derived transcriptome profiling (RNA-seq) in the lung of three tyeps of mice during influenza infection. Methods: Total RNA from lung was extracted using a modified TRIzol protocol and spectrophometrically quantitated. Library preparation and sequencing were conducted using 3’ inTAG next-generation sequencing . Differential gene expression for day 6 post influenza infection was determined relative to mock inoculated mice. Results: Differentially expressed genes were defined using p-value <0.01 and FDR-corrected p-value <0.1 cutoffs. We identified the transcripts in the lung of RIG-I-/-, MAVS -/- mice during influenza infection Our study represents the first detailed analysis of lung transcriptomes of Wild Type , RIG-I-/-, MAVS -/- mice during influenza infection , with biologic replicates, generated by RNA-seq technology.

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ext Generation Sequencing Facilitates Quantitative Analysis of Wild Type and MAP4K1-/- tumor inflitrating T cell Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Methods: Tumor inflitrating T cell mRNA profiles of 21-day after inoculated in wild-type (WT) and Hematopoietic progenitor kinase 1 (HPK1−/−) mice were generated by deep sequencing, in duplicate, using Illumina HiSeq 2500.

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