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E-GEOD-6863

ABSTRACT: Transcription profiling of human immature dendritic cells under hypoxic condition

OTHER RELATED OMICS DATASETS IN: PRJNA99111

PROVIDER: E-GEOD-6863 | ExpressionAtlas | 2015-10-13

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-GEOD-6863-analysis-methods.tsv	Tabular
	E-GEOD-6863-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-6863-configuration.xml	Xml
	E-GEOD-6863-percentile-ranks.tsv	Tabular
	E-GEOD-6863.condensed-sdrf.tsv	Tabular

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Project description:Immature dendritic cells under hypoxic condition

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Transcription profiling of human immature dendritic cells under hypoxic condition

Project description:Dendritic cells (DCs) are professional antigen-presenting cells whose activity is intrinsically linked to the microenvironment. Hypoxia is a condition of low oxygen tension occurring in inflammatory tissues that creates a special microenvironment conditioning cell physiology. We studied the effects of hypoxia on the differentiation of human monocytes into DCs. Immature DCs were differentiated in vitro from human monocytes under normoxic (iDCs) or hypoxic (Hi-DCs) conditions and the gene expression profile was determined. Hi-DCs expressed novel hypoxia-inducible genes and were characterized by up-regulation of pathways associated with cell movement/migration. Experiment Overall Design: The expression profile of immature dendritic cells under normoxia vs hypoxia was studied. Three healthy donors were used as biologial replicates.

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Project description:Dendritic cells (DCs) are professional antigen-presenting cells whose activity is intrinsically linked to the microenvironment. Hypoxia is a condition of low oxygen tension occurring in inflammatory tissues that creates a special microenvironment conditioning cell physiology. We studied the effects of hypoxia on the differentiation of human monocytes into DCs and maturation into mature DCs. Mature DCs were differentiated in vitro from human monocytes under normoxic or hypoxic conditions and the gene expression profile was determined. The expression profile of mature dendritic cells under normoxia vs hypoxia was studied. Three healthy donors were used as biologial replicates.

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