Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-MEXP-2435

ABSTRACT: Transcription profiling by array of Arabidopsis thaliana transgenic plants overexpressing ABF3 under drought stress

PROVIDER: E-MEXP-2435 | ExpressionAtlas | 2015-10-22

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-MEXP-2435-analysis-methods.tsv	Tabular
	E-MEXP-2435-atlasExperimentSummary.Rdata	Rdata
	E-MEXP-2435-configuration.xml	Xml
	E-MEXP-2435-percentile-ranks.tsv	Tabular
	E-MEXP-2435.condensed-sdrf.tsv	Tabular

Items per page:

1 - 5 of 164

Similar Datasets

E-GEOD-23211

Project description:Transcription profiling by array of transgenic rice plants expressing an isopentenyltransferase gene driven by PSARK under water stress

2016-02-16 | E-GEOD-23211 | ExpressionAtlas

Transcription profiling of Arabidopsis thaliana seedlings wild type vs transgenic plants under drought stress

Project description:Plants engineered for abiotic stress tolerance may soon be commercialized. The engineering of these plants typically involves the manipulation of complex multigene networks and may therefore have a greater potential to introduce pleiotropic effects than the simple monogenic traits that currently dominate the plant biotechnology market. Drought- tolerant Arabidopsis thaliana were engineered through overexpression of the transcription factor ABF3 in order to investigate unintended pleiotropic effects. In order to eliminate position effects, the Cre/lox recombination system was used to create control plant lines that contain identical T-DNA insertion sites but with the ABF3 transgene excised. This additionally allowed us to determine if Cre recombinase can cause unintended effects that impact the transcriptome. Microarray analysis of control plant lines that underwent Cre-mediated excision of the ABF3 transgene revealed only two genes that were differentially expressed in more than one plant line, suggesting that the impact of Cre recombinase on the transcriptome was minimal. In the absence of drought stress, overexpression of ABF3 had no effect on the transcriptome, but following drought stress, differences were observed in the gene expression patterns of plants overexpressing ABF3 relative to control plants. Examination of the functional distribution of the differentially expressed genes revealed strong similarity indicating that unintended pathways were not activated. In response to drought stress, overexpression of ABF3 results in a reprogramming of the drought response, which is characterized by changes in the timing or strength of expression of some drought response genes, without activating any unexpected gene networks. These results illustrate that important gene networks are highly regulated in Arabidopsis and that engineering stress tolerance may not necessarily cause extensive changes to the transcriptome.

2010-01-28 | E-MEXP-2435 | biostudies-arrayexpress

E-MEXP-2358

Project description:Transcript profiling of Arabidopsis thaliana transgenic seedlings constitutively overexpressing UGT74E2 (35S::UGT74E2).

2015-10-22 | E-MEXP-2358 | ExpressionAtlas

E-MEXP-2377

Project description:Transcription profiling by array of Arabidopsis srk2cf mutants in a drought stress time course.

2014-09-10 | E-MEXP-2377 | ExpressionAtlas

Gene expression data of OsHMGB707 overexpression and RNAi transgenic rice plants under normal conditions

Project description:High mobility group (HMG) proteins play an important role in regulation of gene transcription through modulate the structure of DNA. In this study, OsHMGB707, a HMG gene localized in rice drought resistance QTL interval, was isolated and the function on rice stress resistance was identified. Overexpression of OsHMGB707 significantly enhanced the drought resistance of the transgenic rice plants, whereas the OsHMGB707-RNAi transgenic rice plants exhibited slightly decrease in drought stress tolerance. To search the downstream genes regulated by OsHMGB707, we performed microarray analysis of the OsHMGB707-overexpressing, OsHMGB707-RNAi and wild-type plants under both normal conditions using Affymetrix Rice Genome Genechip. 21-day-old plants of the OsHMGB707-overexpressing line OE1, OsHMGB707-RNAi line RNAi1 as well as the wild-type plants were used in the normal condition.

2019-05-08 | GSE130821 | GEO

The interaction of mircroRNA171f with SCARECROW-LIKE6-I and II enhances drought stress tolerance through regulation of flavonoid biosynthesis genes in rice

Project description:Plants have evolved a sophisticated defense system to survive under natural drought conditions. MicroRNAs (miRNA) are small noncoding RNAs that act as a post-transcriptional regulator in the environmental stress response and developmental process. Although many studies have reported the involvement of the miRNAs in drought response, molecular mechanisms by which miRNAs confer drought tolerance remain elusive. Here, we show that MIR171f, a member of MIR171 gene family, is mainly expressed in response to drought stress and regulate transcript levels of SCARECROW-LIKE6-I (SCL6-I) and SCL6-II. The SCL6 genes are known to be involved in shoot branching and flag leaf morphology. The MIR171f-overexpressing (MIR171f-OE) transgenic plants showed reduced drought symptoms as compared with non-transgenic (NT) control plants under both field drought and PEG-mediated dehydration stress conditions. Transcriptome analysis using the MIR171f-OE and mir171f-K/O mutants revealed that MIR171f regulates the expression of flavonoid biosynthesis genes, consequently leading to drought tolerance. Flavonoid biosynthesis genes were up-regulated in MIR171f-OE plants as compared with NT control plants under both normal and drought conditions. Together, our findings demonstrated that MIR171f plays an important role in plant drought-tolerance mechanism by regulating transcript levels of SCL6-I and SCL6-II.

2021-07-18 | GSE180271 | GEO

E-MEXP-1383

Project description:Transcription profiling of transgenic mice overexpressing human alpha-synuclein under the PDGF beta promoter to wildtype littermates at 3 months and 9 months of age.

2015-10-22 | E-MEXP-1383 | ExpressionAtlas

E-GEOD-16567

Project description:Transcription profiling by array of seedlings from two maize inbred lines (Ye478 and Han21 )under drought stress, re-watering after drought, or normal watering conditions

2016-10-04 | E-GEOD-16567 | ExpressionAtlas

E-ATMX-20

Project description:Transcription profiling by array of Arabidopsis overexpressing Zat10 under the control of the 35S promoter

2014-07-03 | E-ATMX-20 | ExpressionAtlas

Gene expression data of OsGRAS23 overexpression transgenic rice plants under normal and drought stress conditions

Project description:GRAS transcription factors are plant-specific proteins that play diverse roles in plant development and abiotic stress responses, over-expression of OsGRAS23, a GRAS gene in rice, showed improved drought resistance. To search the downstream genes of OsGRAS23, we performed microarray analysis of the OsGRAS23-overexpressing and wild-type plants under both normal and drought stress conditions using Affymetrix Rice Genome Genechip. 21-day-old plants of the OsGRAS23-overexpressing line OE1 as well as the wild-type plants were used in the drought treatment.

2014-12-31 | GSE64576 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data