Project description:Transcriptional profiling of Arabidopsis transgenic 35S:AtMYB102-SRDX plant under MS medium containing 200mM NaCl for 24h. Transgenic Arabidopsis (background : Col-0) overexpressing AtMYB102 fused to an active transcriptional repression domain (SRDX) showed strong salt tolerance.
Project description:Differential gene expression profiling in ING3-overexpressing MIA PaCa-2 cells was performed using Human Genome U133 Plus 2.0 Array
Project description:Transcriptomic analysis of androgen responsive LNCaP cells depleted (RPS6KB1 KO), overexpressing for RPS6KB1 (RPS6KB1 OE) and treated with berberine (24h treatment with 5 microg/ml prior to radiation) in combination with radiation. Results provide insight into the role of RPS6KB1 in response to radiation.
Project description:Trascriptional profiling by array of bone marrow derived murine cells trasduced with control and Id1-overexpressing vectors to identify genes changes induced by increased expression of the transcriptional regulator Id1
Project description:GFI1 is a transcriptional regulator expressed in lymphoid cells, and an "oncorequisite" factor required for development and maintenance of T-lymphoid leukemia. GFI1 deletion causes hypersensitivity to ionizing radiation, for which the molecular mechanism remains unknown. Here, we demonstrate that GFI1 is required in T cells for the regulation of key DNA damage signalling and repair proteins. Specifically, GFI1 interacts with the arginine methyltransferase PRMT1 and its substrates MRE11 and 53BP1. We demonstrate that GFI1 enables PRMT1 to bind and methylate MRE11 and 53BP1, which is necessary for their function in the DNA damage response. Thus, our results provide evidence that GFI1 can adopt non-transcriptional roles, mediating the post-translational modification of proteins involved in DNA repair. These findings have direct implications for treatment responses in tumours overexpressing GFI1 and suggest that GFI1's activity may be a therapeutic target in these malignancies.
Project description:Transcriptional profiling of rat thyroid cells comparing control untreated thyroid cells with 131I irradiated thyroid cells. Goal was to invesigate radiation induced gene expression profiles