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E-MTAB-5524

ABSTRACT: Transcription profiling by array of rice cell line N1 treated with herbicide safener fenclorim and its analogue CMP

PROVIDER: E-MTAB-5524 | ExpressionAtlas | 2017-03-08

REPOSITORIES: ExpressionAtlas

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Dataset's files

Source:

			Action	DRS
	E-MTAB-5524-analysis-methods.tsv	Tabular
	E-MTAB-5524-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-5524-configuration.xml	Xml
	E-MTAB-5524-percentile-ranks.tsv	Tabular
	E-MTAB-5524.condensed-sdrf.tsv	Tabular

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Fenclorim safening of rice

Project description:The herbicide safener fenclorim is used to protect rice from damage by the herbicide pretilachlor, whilst the structural analogue 4-chloro-6-methyl-2-phenylpyrimidine (CMP) is unable safen rice. Fenclorim and CMP were applied to rice cultures to determine differences in the transcriptional response to a safener and a non-active analogue at four and 24 hours. Rice N1 cell suspension cultures were treated with fenclorim or 4-chloro-6-methyl-2-phenylpyrimidine (CMP) dissolved in acetone to achieve a final concentration of 100uM. The final acetone concentration of 0.1% was replicated in control cell suspension cultures. Samples were taken at four and twenty-four hours post addition in biological triplicate. Suspension cultures were routinely maintained at 25 C in the dark.

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