Project description:DAZAP1 was depleted in culterd HEK 293T cells using shRNA and the resulting poly A RNA were isolated c-DNA library constructed and paired end sequenced on illumina Hi-seq 2000 platform the data was compared to a control shRNA depleted cell
Project description:DAZAP1 was depleted in culterd HEK 293T cells using shRNA and the resulting poly A RNA were isolated c-DNA library constructed and paired end sequenced on illumina Hi-seq 2000 platform the data was compared to a control shRNA depleted cell Gene expression and splicing switches upon DAZAP1 knockdown
Project description:CDCA7L is expressed higher in male astrocytoma/GBM than female tumors or normal cells, and knockdown of CDCA7L blocks growth of male tumor cells, but not female tumor cells. We stably depleted CDCA7L in human GBM cells and compared gene expression to control GBM cells expressing non-trageting scrambled shRNA in male and female cells to identify gene expression differences between male and female cells and between CDCA7L-depleted and scrambled shRNA control cells.
Project description:The goals of this study are to compare mouse oviductal transcriptome profiling (RNA-seq) of PTEN-depleted cells by shRNA versus a scr shRNA control expressing PTEN.
Project description:We analysed whole PolyA+ RNA from human osteosarcoma U2OS cells depleted for human Cactin or transfected with a control shRNA. Two independent shRNAs targeting human Cactin (shCac_C and shCac_D), a control shRNA (shCtrl), a single cell line (U2OS)
Project description:Cdca7l is expressed higher in male astrocytoma/GBM than female tumors or normal cells, and knockdown of Cdca7l blocks growth of male tumor cells, but not female tumor cells. We stably depleted Cdca7l in mouse astrocytoma cells and compared gene expression to control astrocytoma cells expressing non-targeting scrambled shRNA in male and female cells and to wild type primary astrocytes to identify gene expression differences between male and female cells and between Cdca7l-depleted, scrambled shRNA control cells and wild type primary astrocytes.