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E-MTAB-6401

ABSTRACT: Microarray transcriptome profiling of peripheral T-cell lymphomas in two groups differentiated by Twist and Slug protein expression

PROVIDER: E-MTAB-6401 | ExpressionAtlas | 2025-01-23

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-6401-analysis-methods.tsv	Tabular
	E-MTAB-6401-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-6401-configuration.xml	Xml
	E-MTAB-6401-percentile-ranks.tsv	Tabular
	E-MTAB-6401.condensed-sdrf.tsv	Tabular

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Microarray transcriptome profiling of peripheral T-cell lymphomas in two groups differentiated by Twist and Slug protein expression

Project description:This experiment aimed to study the expression of the transcription factors Twist (TWIST1), ZEB1 and Slug (SNAI2) in peripheral T-cell lymphomas, and to see if these markers could be used to delineate lymphomas with different clinical outcomes and genetic profiles. Twist, ZEB1 and Slug expression was studied using immunohistochemistry in 67 Peripheral T-cell lymphomas and significant correlations to progression-free survival were found. Good prognosis group (high Twist, low Slug) and poor prognosis group (low Twist, high Slug) were formed for gene expression profiling. Microarray transcriptome analysis was performed on 12 cases (6 from each prognosis group) to evaluate differences in gene expression between the groups. Total RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tissue blocks using the RNeasy FFPE Kit (Qiagen, Hilden, Germany). Capillary electrophoresis was carried out to check the quality of RNA using the Agilent bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Transcriptome was analysed using GeneChip Human Gene ST Arrays (Affymetrix, Santa Clara, CA, USA) and the WT Pico Kit (Affymetrix Santa Clara, CA, USA) in accordance with the manufacturer’s instructions. Chipster CSC software (ver 3.11) was used to process the data files.

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