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E-MTAB-7062

ABSTRACT: Microarray profiling of mouse pachytene cells deficient for UPF2 or both TUT4 and TUT7

PROVIDER: E-MTAB-7062 | ExpressionAtlas | 2019-01-16

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-MTAB-7062-analysis-methods.tsv	Tabular
	E-MTAB-7062-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-7062-configuration.xml	Xml
	E-MTAB-7062-percentile-ranks.tsv	Tabular
	E-MTAB-7062.condensed-sdrf.tsv	Tabular

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Microarray profiling of mouse pachytene cells deficient for UPF2 or both TUT4 and TUT7

Project description:To study the roles of the proteins TUT4, TUT7 and UPF2 in spermatogenesis, their genes were conditionally deleted in differentiating spermatogonia. Total RNA samples from TUT4 and TUT7-deficient or UPF2-deficient pachytene/diplotene cells were subjected to array profiling.

2019-01-14 | E-MTAB-7062 | biostudies-arrayexpress

Small RNA profiling of TUT4 and TUT7-deficient mouse pachytene-diplotene cells.

Project description:To study the impact of TUT4 and TUT7 on miRNA and piRNA biology, Tut4 and Tut7 were conditionally deleted in differentiating spermatogonia. Total RNA samples from TUT4 and TUT7-deficient pachytene-diplotene cells were used to generate small RNAs libraries.

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Small RNA profiling of TUT4 and TUT7-deficient mouse pachytene-diplotene cells.

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Array profiling of different TUT4 and TUT7 deficient mouse tissues/cells against controls

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MicroRNA profiling of different TUT4 and TUT7 deficient mouse tissues/cells against controls

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Project description:Terminal uridylyl transferases (TUTs) function as integral regulators of microRNA (miRNA) biogenesis by modifying the end structure of precursor miRNA (pre-miRNA). Using biochemistry and deep sequencing techniques, we here investigate the mechanism how human TUT7 recognizes and uridylates pre-miRNAs. We show that the overhang of a pre-miRNA is the key structural element that TUT7 and its paralogues, TUT4 and TUT2, recognize. For group II pre-miRNAs which have a 1 nt 3’ overhang, TUT7 restores the canonical end structure (2 nt 3’ overhang) by mono-uridylation, and thereby promotes miRNA biogenesis. Interestingly, once the 3’ end is receded into the stem (3’ trimmed pre-miRNAs such as Ago-cleaved-pre-miRNA), TUT7 effectively generates an oligo-U tail that consequently leads to degradation. Our single-molecule study further suggests that a distributive mode is employed for both pathways, but the overhang length determines the frequency of TUT7-RNA interaction. Our results explain how TUT7 and TUT4 differentiate pre-miRNA species and reveal a role for TUT7 and TUT4 in the oligo-uridylation and removal of defective pre-miRNAs. HeLa cells were knocked down of control or TUT2/4/7, then total RNAs were prepared for RNA-seq

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