Project description:3'UTR sequencing was performed in indicated C.elegans strains to obtain information about alterations in 3'UTR lengths and alternative polyadenylation events.
Project description:Transcription profiling by high throughput sequencing of leaves of resistant (RG-PtoR) tomato plants treated with different Pst DC3000 mutant strains
Project description:Transcription profiling by high throughput sequencing of Arabidopsis upf1-5 mutant challenged with pathogenic or non-pathogenic Pseudomonas syringae DC3000 strains
Project description:Transcription profiling by high throughput sequencing of oocytes from four inbred mouse strains (129/Sv, C57Bl/6J, C3H/HeN, and DBA/2J)
Project description:Transcription profiling by high throughput sequencing of tomato Rio Grande prf3 leaves challenged with either the flgII-28 peptide or different bacterial strains
Project description:MicroRNAs (miRNAs) play an important role in human brain development and maintenance. To search for miRNAs potentially involved with molecular mechanisms in the pathogenesis of Parkinsons disease (PD), we utilized miRNA microarrays to identify expression changes of 115 annotated Caenorhabditis elegans (C.elegans) miRNAs in human α-synuclein A53T transgenic, dopamine deficient catecholamine transporter gene cat-1 mutant and parkin gene pdr-1 mutant C.elegans strains. Twelve miRNAs were found regulated differentially in α-synuclein transgenic C. elegans, five in cat-1 mutants and three in pdr-1 mutants. The family of miR64/65 appeared co-under-expressed in α-synuclein and cat-1 strains and members of let-7 family co-under-expressed (except miR-241 over-expressed) in a-synuclein and pdr-1 strains. Class H serpentine receptor (srh) family of G-protein-coupled receptor genes were computationally identified to be highly over-represented target candidates for regulated miRNAs.These results indicate that miRNAs are misregulated in C. elegans PD models and suggest a role for these molecules in the disease pathogenesis.
Project description:Analysis of gene expression data in two C.elegans mutant strains: KP3293 tom-1(nu468) and KP3365 unc-43(n1186); hif-1(nu469). These results support the utility of microarray hybridizations to facilitate positional cloning. Keywords: other