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Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots

ABSTRACT: Wound healing in plant tissues, consisting of rigid cell wall-encapsulated cells, represents a considerable challenge and occurs through largely unknown mechanisms distinctive from animals. Owing to their migration inability, plant cells rely on targeted cell division and expansion to regenerate wounds. Strict coordination of these wound-induced responses is essential to ensure efficient, spatially restricted wound healing. Single cell tracking by live imaging allowed us to gain mechanistic insights into the wound perception and coordination of wound-responses after laser-based wounding in Arabidopsis root. We revealed a crucial contribution of the collapse of damaged cells in wound perception and detected an auxin increase specific to cells immediately adjacent to the wound. This localized auxin increase balances wound-induced cell expansion and restorative division rates in a dose-dependent manner, leading to tumorous overproliferation when the canonical TIR1 auxin signalling is disrupted. Auxin and wound-induced turgor pressure changes together also spatially define the activation of key components of regeneration, as the transcription regulator ERF115. Our observations, hence suggest that the wound signalling involves the sensing of collapse of damaged cells and a local auxin signalling activation to coordinate the downstream transcriptional responses in the immediate wound vicinity.

SUBMITTER: Lukas Hoermayer

PROVIDER: S-BIAD23 | bioimages |

REPOSITORIES: bioimages

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Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots.

Project description:Wound healing in plant tissues, consisting of rigid cell wall-encapsulated cells, represents a considerable challenge and occurs through largely unknown mechanisms distinct from those in animals. Owing to their inability to migrate, plant cells rely on targeted cell division and expansion to regenerate wounds. Strict coordination of these wound-induced responses is essential to ensure efficient, spatially restricted wound healing. Single-cell tracking by live imaging allowed us to gain mechanistic insight into the wound perception and coordination of wound responses after laser-based wounding in Arabidopsis root. We revealed a crucial contribution of the collapse of damaged cells in wound perception and detected an auxin increase specific to cells immediately adjacent to the wound. This localized auxin increase balances wound-induced cell expansion and restorative division rates in a dose-dependent manner, leading to tumorous overproliferation when the canonical TIR1 auxin signaling is disrupted. Auxin and wound-induced turgor pressure changes together also spatially define the activation of key components of regeneration, such as the transcription regulator ERF115. Our observations suggest that the wound signaling involves the sensing of collapse of damaged cells and a local auxin signaling activation to coordinate the downstream transcriptional responses in the immediate wound vicinity.

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Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots

Project description: Not available

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Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots

Project description: Not available

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