Supplementary Dataset for "Pore-scale hydrodynamics influence the spatial evolution of bacterial biofilms in a microfluidic porous network"

ABSTRACT: Bacteria occupy heterogeneous environments, attaching and growing within pores in materials, living hosts, and matrices like soil. Systems that permit high-resolution visualization of dynamic bacterial processes within the physical confines of a realistic and tractable porous media environment are rare. Here we use microfluidics to replicate the grain shape and packing density of natural sands in a 2D platform to study the flow-induced spatial evolution of bacterial biofilms underground. We introduce a wildtype strain (Pantoea sp. YR343, n=3) or an EPS-defective strain (Pantoea sp. YR343 ΔUDP, n=3) to the porous media platform and then simulate a rainfall event using gravity-driven flow of bacterial growth media.

ORGANISM(S): Pantoea sp. YR343

SUBMITTER: Jayde Aufrecht 

PROVIDER: S-BSST244 | bioimages |

SECONDARY ACCESSION(S): 23045501

REPOSITORIES: bioimages