Unknown

Dataset Information

0

Mito-QC illuminates mitophagy and mitochondrial architecture in vivo


ABSTRACT: Autophagic turnover of mitochondria, termed mitophagy, is proposed to be an essential quality-control (QC) mechanism of pathophysiological relevance in mammals. However, if and how mitophagy proceeds within specific cellular subtypes in vivo remains unclear, largely because of a lack of tractable tools and models. To address this, we have developed “mito-QC,” a transgenic mouse with a pH-sensitive fluorescent mitochondrial signal. This allows the assessment of mitophagy and mitochondrial architecture in vivo. Using confocal microscopy, we demonstrate that mito-QC is compatible with classical and contemporary techniques in histochemistry and allows unambiguous in vivo detection of mitophagy and mitochondrial morphology at single-cell resolution within multiple organ systems. Strikingly, our model uncovers highly enriched and differential zones of mitophagy in the developing heart and within specific cells of the adult kidney. mito-QC is an experimentally advantageous tool of broad relevance to cell biology researchers within both discovery-based and translational research communities.

SUBMITTER: Thomas G. McWilliams 

PROVIDER: S-JCBD-201603039 | bioimages |

REPOSITORIES: bioimages

Similar Datasets

| S-EPMC4970326 | biostudies-literature
| S-EPMC7932697 | biostudies-literature
| S-EPMC4874835 | biostudies-literature
| S-EPMC10452872 | biostudies-literature
| S-EPMC3230385 | biostudies-literature
| S-EPMC3278611 | biostudies-literature
| S-EPMC3673629 | biostudies-literature
| S-EPMC2278073 | biostudies-literature
| S-EPMC6762132 | biostudies-literature
| S-EPMC7247689 | biostudies-literature