Dataset Information

Curien2003_MetThr_synthesis

ABSTRACT: This a model from the article: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana. Curien G, Ravanel S, Dumas R Eur. J. Biochem. 2003 Dec; Volume: 270 (Issue: 23 )]:4615-27 14622248 , Abstract: This work proposes a model of the metabolic branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana which involves kinetic competition for phosphohomoserine between the allosteric enzyme threonine synthase and the two-substrate enzyme cystathionine gamma-synthase. Threonine synthase is activated by S-adenosylmethionine and inhibited by AMP. Cystathionine gamma-synthase condenses phosphohomoserine to cysteine via a ping-pong mechanism. Reactions are irreversible and inhibited by inorganic phosphate. The modelling procedure included an examination of the kinetic links, the determination of the operating conditions in chloroplasts and the establishment of a computer model using the enzyme rate equations. To test the model, the branch-point was reconstituted with purified enzymes. The computer model showed a partial agreement with the in vitro results. The model was subsequently improved and was then found consistent with flux partition in vitro and in vivo. Under near physiological conditions, S-adenosylmethionine, but not AMP, modulates the partition of a steady-state flux of phosphohomoserine. The computer model indicates a high sensitivity of cystathionine flux to enzyme and S-adenosylmethionine concentrations. Cystathionine flux is sensitive to modulation of threonine flux whereas the reverse is not true. The cystathionine gamma-synthase kinetic mechanism favours a low sensitivity of the fluxes to cysteine. Though sensitivity to inorganic phosphate is low, its concentration conditions the dynamics of the system. Threonine synthase and cystathionine gamma-synthase display similar kinetic efficiencies in the metabolic context considered and are first-order for the phosphohomoserine substrate. Under these conditions outflows are coordinated. SBML level 2 code generated for the JWS Online project by Jacky Snoep using PySCeS Run this model online at http://jjj.biochem.sun.ac.za To cite JWS Online please refer to: Olivier, B.G. and Snoep, J.L. (2004) Web-based modelling using JWS Online , Bioinformatics, 20:2143-2144 Biomodels Curation The model simulates the flux for TS and CGS under conditions given in Table 2 and reproduces the dotted lines given in Table 3 of the paper. There is a typo in the equation for the apparent specificity constant for Phser, Kts (equation13). This was changed after communication with the authors to be: Kts = 5.9E-4+6.2E-2*pow(AdoMet,2.9)/(pow(32,2.9)+pow(AdoMet,2.9)). The model was successfully tested on Jarnac and Copasi. Due to a suggestion from Pedro Mendez the parameter AdoMet, TS and CGS where made constant species. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2010 The BioModels.net Team. For more information see the terms of use . To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.

SUBMITTER: Nicolas Le Novère

PROVIDER: BIOMD0000000068 | BioModels | 2024-09-02

REPOSITORIES: BioModels

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			Action	DRS
	BIOMD0000000068?filename=BIOMD0000000068-biopax2.owl	Owl
	BIOMD0000000068?filename=BIOMD0000000068-biopax3.owl	Owl
	BIOMD0000000068?filename=BIOMD0000000068-matlab.m	Other
	BIOMD0000000068?filename=BIOMD0000000068-octave.m	Other
	BIOMD0000000068?filename=BIOMD0000000068.m	Other

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A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana.

Curien Gilles G Ravanel Stéphane S Dumas Renaud R

European journal of biochemistry 20031201 23

This work proposes a model of the metabolic branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana which involves kinetic competition for phosphohomoserine between the allosteric enzyme threonine synthase and the two-substrate enzyme cystathionine gamma-synthase. Threonine synthase is activated by S-adenosylmethionine and inhibited by AMP. Cystathionine gamma-synthase condenses phosphohomoserine to cysteine via a ping-pong mechanism. Reactions are irrever ...[more]

PMID: 14622248

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