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Aubry1995 - Multi-compartment model of fluid-phase endocytosis kinetics in Dictyostelium discoideum

ABSTRACT: Fluid-phase endoeytosis (pinocytosis) kinetics were studied in Dictyostelium discoideum amoebae from the axenic strain Ax-2 that exhibits high rates of fluid-phase endoeytosis when cultured in liquid nutrient media. Fluorescein-labelled dextran (FITC-dextran) was used as a marker in continuous uptake- and in pulse-chase exocytosis experiments. In the latter case, efflux of the marker was monitored on cells loaded for short periods of time and resuspended in marker-free medium. A multicompartmental model was developed which describes satisfactorily fluid-phase endocytosis kinetics. In particular, it accounts correctly for the extended latency period before exocytosis in pulse-chase experiments and it suggests the existence of some sorts of maturation stages in the pathway.

SUBMITTER: Kausthubh Ramachandran

PROVIDER: BIOMD0000000986 | BioModels | 2024-09-02

REPOSITORIES: BioModels

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			Action	DRS
	BIOMD0000000986?filename=Aubry1995-biopax2.owl	Owl
	BIOMD0000000986?filename=Aubry1995-biopax3.owl	Owl
	BIOMD0000000986?filename=Aubry1995-matlab.m	Other
	BIOMD0000000986?filename=Aubry1995-octave.m	Other
	BIOMD0000000986?filename=Aubry1995.cps	Other

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Aubry1995 - Nine-compartment model of fluid-phase endocytosis kinetics in Dictyostelium discoideum

Project description:Fluid-phase endoeytosis (pinocytosis) kinetics were studied in Dictyostelium discoideum amoebae from the axenic strain Ax-2 that exhibits high rates of fluid-phase endoeytosis when cultured in liquid nutrient media. Fluorescein-labelled dextran (FITC-dextran) was used as a marker in continuous uptake- and in pulse-chase exocytosis experiments. In the latter case, efflux of the marker was monitored on cells loaded for short periods of time and resuspended in marker-free medium. A multicompartmental model was developed which describes satisfactorily fluid-phase endocytosis kinetics. In particular, it accounts correctly for the extended latency period before exocytosis in pulse-chase experiments and it suggests the existence of some sorts of maturation stages in the pathway.

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