Dataset Information

Senger2008 - Genome-scale metabolic network of Clostridium acetobutylicum (iCac802)

ABSTRACT: Senger2008 - Genome-scale metabolic network of Clostridium acetobutylicum (iCac802) This model is described in the article: Genome-scale model for Clostridium acetobutylicum: Part I. Metabolic network resolution and analysis. Senger RS, Papoutsakis ET. Biotechnol. Bioeng. 2008 Dec; 101(5): 1036-1052 Abstract: A genome-scale metabolic network reconstruction for Clostridium acetobutylicum (ATCC 824) was carried out using a new semi-automated reverse engineering algorithm. The network consists of 422 intracellular metabolites involved in 552 reactions and includes 80 membrane transport reactions. The metabolic network illustrates the reliance of clostridia on the urea cycle, intracellular L-glutamate solute pools, and the acetylornithine transaminase for amino acid biosynthesis from the 2-oxoglutarate precursor. The semi-automated reverse engineering algorithm identified discrepancies in reaction network databases that are major obstacles for fully automated network-building algorithms. The proposed semi-automated approach allowed for the conservation of unique clostridial metabolic pathways, such as an incomplete TCA cycle. A thermodynamic analysis was used to determine the physiological conditions under which proposed pathways (e.g., reverse partial TCA cycle and reverse arginine biosynthesis pathway) are feasible. The reconstructed metabolic network was used to create a genome-scale model that correctly characterized the butyrate kinase knock-out and the asolventogenic M5 pSOL1 megaplasmid degenerate strains. Systematic gene knock-out simulations were performed to identify a set of genes encoding clostridial enzymes essential for growth in silico. This model is hosted on BioModels Database and identified by: MODEL1507180027. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

SUBMITTER: Nicolas Le Novère

PROVIDER: MODEL1507180027 | BioModels | 2015-07-30

REPOSITORIES: BioModels

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	MODEL1507180027?filename=MODEL1507180027-biopax2.owl	Owl
	MODEL1507180027?filename=MODEL1507180027-biopax3.owl	Owl
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Genome-scale model for Clostridium acetobutylicum: Part I. Metabolic network resolution and analysis.

Senger Ryan S RS Papoutsakis Eleftherios T ET

Biotechnology and bioengineering 20081201 5

A genome-scale metabolic network reconstruction for Clostridium acetobutylicum (ATCC 824) was carried out using a new semi-automated reverse engineering algorithm. The network consists of 422 intracellular metabolites involved in 552 reactions and includes 80 membrane transport reactions. The metabolic network illustrates the reliance of clostridia on the urea cycle, intracellular L-glutamate solute pools, and the acetylornithine transaminase for amino acid biosynthesis from the 2-oxoglutarate p ...[more]

PMID: 18767192

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