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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of wild type and C/EBPbeta deficient mice during a partial hepatectomy time series to identify transcriptional targets of C/EBPbeta

ABSTRACT: The aim of this experiment was to use microarray analysis to compare the response of wild type (WT) and C/EBPbeta deficient (KO) mice during a partial hepatectomy time course in hopes of identifying transcriptional targets of C/EBPbeta. In addition, the WT time course alone was examined to analyze mammalian cellular proliferation in vivo. In the partial hepatectomy model, quiescent hepatocytes reenter the cell cycle and progress in a synchronous fashion. This allows for the elucidation of regulatory networks operative in mammalian cell cycle. (Identification of transcriptional networks during liver regeneration (2004) Journal of Biological Chemistry)

ORGANISM(S): Mus musculus

SUBMITTER: Peter White

PROVIDER: E-CBIL-16 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Orthogonal analysis of C/EBPbeta targets in vivo during liver proliferation.

Friedman Joshua R JR Larris Brian B Le Phillip P PP Peiris T Harshani TH Arsenlis Athanasios A Schug Jonathan J Tobias John W JW Kaestner Klaus H KH Greenbaum Linda E LE

Proceedings of the National Academy of Sciences of the United States of America 20040818 35

CCAAT enhancer-binding protein beta (C/EBPbeta), a basic-leucine zipper transcription factor, is an important effector of signals in physiologic growth and cancer. The identification of direct C/EBPbeta targets in vivo has been limited by functional compensation by other C/EBP family proteins and the low stringency of the consensus sequence. Here we use the combined power of expression profiling and high-throughput chromatin immunoprecipitation to identify direct and biologically relevant target ...[more]

PMID: 15317935

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