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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human pancreatic islets from normal and Type 2 diabetic subjects

ABSTRACT: Human pancreatic islets isolated from 7 people with normal glucose tolerance, and 5 people with type 2 diabetes. All 12 people were organ donors after either cerebrovascular accident or intracerebral haemorrhage. Normals were required to maintain glucose at least 6.1mM in intensive care. Diabetic subjects were all at least 10 years from diagnosis and not insulin-requiring. For every subject, RNA was isolated, cRNA was made and hybridized to the U133A and U133B Affymetrix arrays (total of 24 arrays). No samples were pooled.

ORGANISM(S): Homo sapiens

DISEASE(S): diabetes mellitus

SUBMITTER: Ronald Kahn

PROVIDER: E-CBIL-20 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Loss of ARNT/HIF1beta mediates altered gene expression and pancreatic-islet dysfunction in human type 2 diabetes.

Gunton Jenny E JE Kulkarni Rohit N RN Yim SunHee S Okada Terumasa T Hawthorne Wayne J WJ Tseng Yu-Hua YH Roberson Russell S RS Ricordi Camillo C O'Connell Philip J PJ Gonzalez Frank J FJ Kahn C Ronald CR

Cell 20050801 3

beta cell dysfunction is a central component of the pathogenesis of type 2 diabetes. Using oligonucleotide microarrays and real-time PCR of pancreatic islets isolated from humans with type 2 diabetes versus normal glucose-tolerant controls, we identified multiple changes in expression of genes known to be important in beta cell function, including major decreases in expression of HNF4alpha, insulin receptor, IRS2, Akt2, and several glucose-metabolic-pathway genes. There was also a 90% decrease i ...[more]

PMID: 16096055

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