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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The role of CpG island methylation in immune cell gene regulation

ABSTRACT: Tissue-specific methylation patterns suggest a role for CpG island methylation in differentiation and cell-type-specific gene regulation. We have profiled CpG island methylation in different cells of the immune cell lineage to investigate this role. MBD-affinity purification combined with next generation sequencing was used to analyse CpG island methylation in dendritic cells, B cells, Th1, Th2 and naïve T cells. ChIP-seq was carried out to determine RNA polymerase II binding sites in these cell types and this was compared to the methylation profiles obtained. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ Abstract: We have profiled CpG island methylation in various immune system cell types and related this to gene expression in these cells.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Mus musculus

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PROVIDER: E-ERAD-27 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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