Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

33 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Kristen-PAO1-aerobic PA0779

ABSTRACT: To see the effect of lethal contrations of tobramycin under aerobic conditions in PAO1::PA0779 . RNA was isolated from 3 biological repeats of PAO1::PA0779 grown to mid-log in cationic adjusted meuller hinton broth containing 15mM KNO3, and 3 biological repeats of PAO1::PA0779 to which 2 ug/ml tobramycin was added after cells reached mid-log.

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Kristen Kindrachuck

PROVIDER: E-FPMI-27 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Kristen-PAO1-anaerobic sub-lethal concentrations tobramycin

Project description:To see the effect of sub-lethal concentration of tobramcyin on PAO1 under anaerobic conditions. RNA was isolated from 5 biological repeats of PAO1 grown to mid-log phase in cationic adjusted mueller hinton broth containing 15mM KNO3 and 5 biological repeats of PAO1 grown to mid-log phase in cationic adjusted mueller hinton broth containing 15mM KNO3 and then treated for 30 minutes with 2 ug/ml tobramycin. All flasks were sealed to create anaerobic conditions. null

2011-01-20 | E-FPMI-25 | biostudies-arrayexpress

Kristen-PAO1 aerobic lethal concentrations of Tobramycin

Project description:To see the effect of lethal concentrations of tobramycin on Pseudomonas aeruginosa grown under aerobic conditions. RNA was isolated from 4 biological repeats of P.aeruginosa grown to mid-log in cationic adjusted mueller hinton broth containing 15mM KNO3 and 4 biological repeats of P.aeruginosa grown to mid-log in cationic adjusted mueller hinton broth containing 15mM KNO3 and treated with 2 ug/ml tobramycin for 30 minutes. null

2011-01-20 | E-FPMI-26 | biostudies-arrayexpress

Kristen-PAO1-anaerobic lethal concentrations tobramycin

Project description:To see the effects of tobramycin on Pseudomonas aeruginosa under anaerboc lethal conditions. RNA was isolated from 5 biological repeats of P.aeruginosa grown in cationic adjusted mueller hinton broth containing 15mM KNO3 and 5 biological repeats of P.aeruginosa grown in cationic adjusted mueller hinton broth containing 15mM KNO3 after being treated with 20 ug/ml tobramycin for 30 minutes.

2011-01-20 | E-FPMI-24 | biostudies-arrayexpress

Kristen-PAO1 aerobic sub-inhibitory cond. tobramycin

Project description:To see the effect of sub-inhibitory concentrations of tobramycin on Pseudomonas aeruginosa grown under aerobic conditions. RNA was isolated form 4 biological repeats of P.aeruginosa grown to mid-log in cationic adjusted mueller hinton broth and 4 biological repeats of P.aeruginosa with the addition of 0.25ug/ml tobramycin in cationic adjusted mueller hinton broth. null

2011-03-30 | E-FPMI-23 | biostudies-arrayexpress

Transcription profiling of P. aeruginosa PA01 grown with or without human LL37

Project description:Five biological repeats of P.aeruginosa PAO1 in the absence of human LL37 and five biological repeats of P.aeruginosa presence of LL37 were grown in mineral medium in continuous-culture flow cells. Microarray experiments were done using microarray slides and protocols from TIGR on cells harvested from the biofilms after 4 days of growth.

2008-10-25 | E-FPMI-12 | biostudies-arrayexpress

Transcription profiling of P.aeruginosa PAO1 and PAO1 phoQ::xylE

Project description:Five biological repeats of P.aeruginosa PAO1 and five biological repeats of P.aeruginosa PAO1 phoQ::xylE were grown in mineral medium BM2 using glucose as the carbon source and with 2mM MgSO4. Microarray experiments were done using microarray slides and protocols from TIGR on cells from mid-log phase.

2008-12-20 | E-FPMI-18 | biostudies-arrayexpress

Transcription profiling of Pseudomonas aeruginosa parental strain PAO1 and PAOQ_psrA::Tn5 knock out mutant

Project description:5 Biological repeats of bacterial strain PAO1(parent, control) were compared to 5 biological repeats of PAO1_psrA::Tn5 knockout mutant, both strains were grown in BM2-glucose media containing 2mM MgSO4. Cells were grown to mid-log phase, OD600 0.50 Cells were immediately harvested and RNA was extracted

2008-07-04 | E-FPMI-14 | biostudies-arrayexpress

Transcription profiling of Pseudomonas aeruginosa strain PA01 grown in swimming of swarming conditions

Project description:Microarray were done on 4 independent cultures. PA01 was grown for 6 hours to late log growth phase (OD600 0.9-1.0) in liquid BM2-swarming media under shaking conditions(swimming) or for 18 hours at 37C on BM2-swarming plate containing 0.5% (w/v) agar and 0.1% casamino acids. Cells were harvested either by centrifugation(swimming) or by a sterile inoculating loop (swarming) and were resuspended n BM2-swarming media supplement with RNAprotect reagent (Qiagen)

2008-03-01 | E-FPMI-10 | biostudies-arrayexpress

Transcription profiling of P.aeruginosa PA14 and P.aeruginosa PA14 metR::phoA

Project description:Five biological repeats of P.aeruginosa PA14 and five biological repeats of P.aeruginosa PA14 metR::phoA were grown in liquid BM2 swarming media containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, and 0.1% (wt/vol) casamino acids. Microarray experiments were done using microarray slides and protocols from TIGR on samples taken from mid-log phase (OD600 0.4-0.5)cells.

2009-08-20 | E-FPMI-19 | biostudies-arrayexpress

Transcription profiling of P.aeruginosa PA14 and P.aeruginosa PA14 metR::phoA grown on swarming plates

Project description:Four biological repeats of P.aeruginosa PA14 and four biological repeats of P.aeruginosa PA14 metR::phoA were grown in BM2 swarming media plates containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, 0.1% (wt/vol) casamino acids and 0.5% agar. Microarray experiments were done using microarray slides and protocols from TIGR on samples harvested from the dendritic swarm colony growth, 2 to 3 mm of the swarm zone edge.

2009-08-20 | E-FPMI-20 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data