Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of peripheral blood monocytes from healthy humans and from those with Chronic Granulamatous Disease in response to stimulation with peptidoglycan and lipopolysaccharide

ABSTRACT: Persons with Chronic Granulomatous Disease, are susceptible to a narrow range of infections resulting from the failure of cells to generate toxic reactive oxygen species (ROS) required for phagocytice oxidative killing of microorganisms. For unknown reasons, many inflammatory conditions (inflammatory bowel disease, periodontal inflammation, granulomatous obstruction of the urinary and gastrointestinal tract and “sterile” inflammation of the lungs and other organs) are also associated with the disease. The goal of this study is to investigate the role of ROS in the regulation of inflammatory and immunity genes induced by Toll-like Receptors (TLRs). Transcriptional responses to peptidoglycan or lipopolysaccharide (TLR2/4 agonists) were evaluated at 4 and 24 hrs in peripheral blood monocytes (PBM) from three males with CGD compared to PBM from 5 healthy individuals.

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Homo sapiens

DISEASE(S): chronic granulomatous disease

SUBMITTER: Karsten Hokamp 

PROVIDER: E-FPMI-9 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

ROS-deficient monocytes have aberrant gene expression that correlates with inflammatory disorders of chronic granulomatous disease.

Brown Kelly L KL   Bylund Johan J   MacDonald Kelly L KL   Song-Zhao George X GX   Elliott Melissa R MR   Falsafi Reza R   Hancock Robert E W RE   Speert David P DP  

Clinical immunology (Orlando, Fla.) 20080726 1

Chronic granulomatous disease is an immunodeficiency caused by an inability to produce reactive oxygen species. While the mechanism of hyper-sensitivity to infection is well understood in CGD, the basis for debilitating inflammatory disorders that arise in the absence of evident infection has not been fully explained. Herein it is demonstrated that resting and TLR-activated monocytes from individuals with CGD expressed significantly higher levels of inflammatory mediators than control cells; the  ...[more]

Similar Datasets

Transcriptomics Transcription profiling of responses to TNF-alpha, IL-1beta, peptidoglycan or lipopolysaccharide (TLR2/4 agonists) evaluated at 4 hrs in peripheral blood monocytes (PBM) from the patient bearing the IRAK4 Q293X mutation vs PBM from 5 healthy individuals
2010-05-19 | E-FPMI-7 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human CD14+ monocytes treated with innate defence regulator peptide IDR-1 in the presence/absence of lipopolyssacharide
2007-04-10 | E-FPMI-8 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human monocytic cells THP-1 stimulated with LPS (10ng/ml) in presence or absence of LL-37 (5 ug/ml), as well as with the peptide alone, for 4 hours
2006-12-07 | E-FPMI-6 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human bronchial epithelial cells stimulated with the commensal bacterium S. salivarius K12 of the pathogens S. aureus, P. aeruginosa, or S. enteritidis
2008-10-30 | E-FPMI-13 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human monocytic cell line, THP-1 cells were stimulated 4 hours with chemokine MIG or CCL2/MCP-1
2010-07-01 | E-FPMI-5 | biostudies-arrayexpress
Transcriptomics Transcription profiling of endotoxin-stimulated human monocytic cells in presence and absence of host defense peptide LL-37
2006-08-01 | E-FPMI-4 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Aspergillus fumigatus cultures using RNA subjected to 1, 2 or no rounds of amplifciation to identify amplification protocol dependent differences in log ratios of intensity
2008-01-10 | E-TABM-330 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Aspergillus fumigatus isolates following laboratory culture or initiation of infection in the neutropenic murine lung to understand how metabolic and nutritional factors governing adaptation to the host niche contribute to virulence
2008-03-31 | E-TABM-327 | biostudies-arrayexpress
no omics defined Transcription profiling of maize to identify gene expression changes after chilling for 28 hours (acclimation phase)
2009-11-01 | E-MEXP-1865 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human carcinoma in situs cells from testis compared to fetal gonocytes, oogonia and cultured embryonic stem cells
2009-06-15 | E-TABM-488 | biostudies-arrayexpress