Unknown,Transcriptomics,Genomics,Proteomics

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ZzMex67 co-IPed RNA vs. Total RNA (zzMex67IP_v_TotalRNA)

ABSTRACT: Comparison of cDNA from RNA IPed with zzMex67 to that from total RNA; resulting analysis gives Mex67 binding level relative to total abundance for each mRNA

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Pamela Silver 

PROVIDER: E-GEOD-100 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide analysis of RNA-protein interactions illustrates specificity of the mRNA export machinery.

Hieronymus Haley H   Silver Pamela A PA  

Nature genetics 20030113 2

Nuclear export of mRNA is mediated by a complex machinery of RNA-binding proteins that recognizes and routes mRNAs through a messenger ribonucleoprotein (mRNP) network. The full spectrum of mRNA cargoes for any dedicated mRNA export factor is unknown. We identified the mRNAs that bind two conserved yeast mRNA export factors, Yra1 (refs. 1-5) and Mex67 (refs. 6,7), on a genome-wide scale and determined their level of binding. Yra1 and Mex67 bind approximately 1,000 and 1,150 mRNAs, respectively,  ...[more]

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