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Transcription profiling of mouse liver samples comparing hormone sensitive lipase (HSL) knockout mice vs. wildtype

ABSTRACT: HSL is a key enzyme in in the mobilization of fatty acids from the triglyceride stores of white adipose tissue. In addition, it is expressed in mice liver. In the present microarray study, changes in the transcript profile of murine liver samples due to global HSL knockout were investigated. Experiment Overall Design: Genetic modification to analyze the impact of a general knockout of the HSL gene on liver metabolism. Experiment Overall Design: HSL knockout (ko) mice versus wildtype (wt) mice. Mice fed a normal diet (ND) versus mice fed a high fat diet (FD) for 6 months. Equal amounts of liver samples from six mice were pooled and total RNA was extracted, except for HSL-null mice on FD were only 4 livers were pooled.

ORGANISM(S): Mus musculus

SUBMITTER: CÃ©line Fernandez

PROVIDER: E-GEOD-10067 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Disturbed cholesterol homeostasis in hormone-sensitive lipase-null mice.

Fernandez Céline C Lindholm Marie M Krogh Morten M Lucas Stéphanie S Larsson Sara S Osmark Peter P Berger Karin K Borén Jan J Fielding Barbara B Frayn Keith K Holm Cecilia C

American journal of physiology. Endocrinology and metabolism 20080729 4

Transcriptomics analysis revealed that genes involved in hepatic de novo cholesterol synthesis were downregulated in fed HSL-null mice that had been on a high-fat diet (HFD) for 6 mo. This finding prompted a further analysis of cholesterol metabolism in HSL-null mice, which was performed in fed and 16-h-fasted mice on a normal chow diet (ND) or HFD regimen. Plasma cholesterol was elevated in HSL-null mice, in all tested conditions, as a result of cholesterol enrichment of HDL and VLDL. Hepatic e ...[more]

PMID: 18664600

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