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AhR activation by TCDD in the ET, cortical epithelial cell line

ABSTRACT: Over activation of the aryl hydrocarbon receptor (AhR) by TCDD results ampng other phenotypes in severe thymic atrophy accompanied by immunosuppression. The link between thymic atrophy, skewed thymocyte differntiation and immunosuppression is still not fully resolved. This study investigates the TCDD elicted exprssion changes in the ET, cortical thymus epithelial cell line. Keywords: TCDD, AhR, thymic epithelial cells, thymic involution, thymus atrophy ET cells were grown to 80-90% confluence and treated with 5nM TCDD or solvent control (1,4-Dioxane, 0,05%V). 2h,4h and 6h after treatment, RNA was isolated using TRIzol and gene expression measured. All experiments wer done in biological duplicates.

ORGANISM(S): Mus musculus

SUBMITTER: Tim Zacharewski

PROVIDER: E-GEOD-10168 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Promoter analysis of TCDD-inducible genes in a thymic epithelial cell line indicates the potential for cell-specific transcription factor crosstalk in the AhR response.

Frericks Markus M Burgoon Lyle D LD Zacharewski Timothy R TR Esser Charlotte C

Toxicology and applied pharmacology 20080719 2

Activation of the aryl hydrocarbon receptor (AhR(1)) by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) elicits severe immunosuppression accompanied by thymic atrophy. Previous evidence suggests that TCDD targets both thymocytes and thymic epithelial cells. The AhR induces cell-specific changes in gene transcription via binding to the dioxin response element DRE; however, the underlying specificity-mechanisms, in particular with regard to the role of promoter element context, and possible transcripti ...[more]

PMID: 18691609

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