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Transcription profiling of mouse epidermis of Tcfap2c mutant and control skin at embryonic day 16.5

ABSTRACT: The development of the epidermis, a stratified squamous epithelium, is dependent on the regulated differentiation of keratinocytes. Differentiation begins with the initiation of stratification, a process tightly controlled through proper gene expression. AP-2Î³ is expressed in skin and previous research suggested a pathway where p63 gene induction results in increased expression of AP-2Î³ which in turn is responsible for induction of K14. This study uses a conditional gene ablation model to further explore the role of AP-2Î³ in skin development. Mice deficient for AP-2Î³ exhibited delayed expression of p63, K14, and K1, key genes required for development and differentiation of the epidermis. In addition, microarray analysis of E16.5 skin revealed delayed expression of additional late epidermal differentiation genes: filaggrin, repetin and secreted Ly6/Plaur domain containing 1, in mutant mice. The genetic delay in skin development was further confirmed by a functional delay in the formation of an epidermal barrier. These results document an important role for AP-2Î³ in skin development, and reveal the existence of regulatory factors that can compensate for AP-2Î³ in its absence. Experiment Overall Design: Skin samples were collected from 3 Tcfap2c and 3 control animals at embryonic day 16.5.

ORGANISM(S): Mus musculus

SUBMITTER: Jill Guttormsen

PROVIDER: E-GEOD-10175 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparison of gene expression in the epidermis of Tcfap2c mutant and control skin at embryonic day 16.5

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