Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

High-throughput pyrosequencing of endogenous small RNAs from Arabidopsis thaliana wild-type and polIV mutants

ABSTRACT: Comparison of the endogenous small RNA content of Arabidopsis flower bud tissue: wild type vs. mutants in polIV pathways Keywords: High throughput 454 small RNA sequencing. Size fractionated small RNA from total RNA extracts was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to 454 high throughput pyrosequencing. Please see www.454.com for details of the sequencing technology.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Frank Schwach

PROVIDER: E-GEOD-10180 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

PolIVb influences RNA-directed DNA methylation independently of its role in siRNA biogenesis.

Mosher Rebecca A RA Schwach Frank F Studholme David D Baulcombe David C DC

Proceedings of the National Academy of Sciences of the United States of America 20080219 8

DNA-dependent RNA polymerase (Pol)IV in Arabidopsis exists in two isoforms (PolIVa and PolIVb), with NRPD1a and NRPD1b as their respective largest subunits. Both isoforms are implicated in production and activity of siRNAs and in RNA-directed DNA methylation (RdDM). Deep sequence analysis of siRNAs in WT Arabidopsis flowers and in nrpd1a and nrpd1b mutants identified >4,200 loci producing siRNAs in a PolIV-dependent manner, with PolIVb reinforcing siRNA production by PolIVa. Transposable element ...[more]

PMID: 18287047

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Dataset Information

High-throughput pyrosequencing of endogenous small RNAs from Arabidopsis thaliana wild-type and polIV mutants

Publications

PolIVb influences RNA-directed DNA methylation independently of its role in siRNA biogenesis.

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