Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Human colon expression in healthy controls and UC


ABSTRACT: Activation of inflammatory pathways in human IBD. Leukocyte recruitment pathways including those for eosiniphils are activated in the affected colon in IBD. However, the functional implications of this are not known. We hypothesized that pro-inflammatory eotaxin (CCL11) dependent networks would be up regulated in the colon of pediatric patients with Ulcerative Colitis (UC), and that these would regulate eosinophil recruitment to the gut. These experiments tested differential colon gene expression relative to these pathways in healthy and UC samples. Colon biopsy samples were obtained from UC patients at diagnosis, and healthy controls. The global pattern of gene expression was determined using GeneSpring software, and biological networks were identified using Ingenuity software. Data suggested that a leukocyte recruitment network which includeds CCL11 is up regulated in pediatric UC at diagnosis. The degree of up regulation of these genes compared to healthy controls was remarkably conserved within the UC patient group, suggesting common mechanisms of mucosal inflammation. These experiments tested differential colon gene expression relative to these pathways in healthy and UC samples. Keywords: Single time point in UC and healthy controls. Colon RNA was isolated from biopsies obtained from UC at diagnosis and healthy controls. Samples were obtained from the most proximal affected segment of colon. Microarray experiments were performed as described in the CCHMC microarray core, and data was analyzed as described above in the summary. The '107' internal control CEL files (for batches 1,2,4,5) used for ormalization of the Sample VALUEs are also contained within this data set.

ORGANISM(S): Homo sapiens

SUBMITTER: Lee Denson 

PROVIDER: E-GEOD-10191 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Intestinal macrophage/epithelial cell-derived CCL11/eotaxin-1 mediates eosinophil recruitment and function in pediatric ulcerative colitis.

Ahrens Richard R   Waddell Amanda A   Seidu Luqman L   Blanchard Carine C   Carey Rebecca R   Forbes Elizabeth E   Lampinen Maria M   Wilson Tara T   Cohen Elizabeth E   Stringer Keith K   Ballard Edgar E   Munitz Ariel A   Xu Huan H   Lee Nancy N   Lee James J JJ   Rothenberg Marc E ME   Denson Lee L   Hogan Simon P SP  

Journal of immunology (Baltimore, Md. : 1950) 20081101 10


Clinical studies have demonstrated a link between the eosinophil-selective chemokines, eotaxins (eotaxin-1/CCL11 and eotaxin-2/CCL24), eosinophils, and the inflammatory bowel diseases, Crohn's disease and ulcerative colitis (UC). However, the cellular source and individual contribution of the eotaxins to colonic eosinophilic accumulation in inflammatory bowel diseases remain unclear. In this study we demonstrate, by gene array and quantitative PCR, elevated levels of eotaxin-1 mRNA in the rectos  ...[more]

Similar Datasets

2008-12-22 | GSE10191 | GEO
2010-05-27 | E-GEOD-9686 | biostudies-arrayexpress
2008-01-01 | GSE9686 | GEO
2009-03-10 | E-GEOD-10616 | biostudies-arrayexpress
2009-03-11 | GSE10616 | GEO
2023-10-27 | GSE222070 | GEO
2016-01-28 | GSE72221 | GEO
2015-04-10 | E-GEOD-67106 | biostudies-arrayexpress
2012-04-19 | E-GEOD-37283 | biostudies-arrayexpress
2019-09-01 | E-MTAB-7915 | biostudies-arrayexpress