Unknown,Transcriptomics,Genomics,Proteomics

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Desiccation and control transcriptomes of Rhodococcus jostii RHA1

ABSTRACT: Here we report the first transcriptomic analysis of a Gram-positive bacterium to desiccation. Filtered RHA1 cells incubated at either low relative humidity (20%), as an air drying treatment, or high relative humidity (100%), as a control, were transcriptionally profiled over a comprehensive time series. Keywords: stress response time course Three biological replicates from both the desiccation and control experiments were analyzed using two-colour microarrays. Over a time series for each experiment, Cy-labelled cDNA from treated and time-zero cells were hybridized, with a dye swap for one of the replicates.

ORGANISM(S): Rhodococcus sp. RHA1

SUBMITTER: William Mohn 

PROVIDER: E-GEOD-10378 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Global response to desiccation stress in the soil actinomycete Rhodococcus jostii RHA1.

LeBlanc Justin C JC   Gonçalves Edmilson R ER   Mohn William W WW  

Applied and environmental microbiology 20080307 9

Rhodococcus jostii RHA1 is a soil-residing actinomycete with many favorable metabolic capabilities that make it an ideal candidate for the bioremediation of contaminated soils. Arguably the most basic requirement for life is water, yet some nonsporulating bacteria, like RHA1, can survive lengthy droughts. Here we report the first transcriptomic analysis of a gram-positive bacterium during desiccation. Filtered RHA1 cells incubated at either low relative humidity (20%), as an air-drying treatment  ...[more]

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