Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human skin from patients with acne before and 1 week after isotretinoin (13-cis retinoic acid) treatment


ABSTRACT: The pathogenesis of acne has been linked to multiple factors such as increased sebum production, inflammation, follicular hyperkeratinization, and the action of Propionibacterium acnes within the follicle. 13-cis Retinoic Acid (13-cis RA, isotretinoin) is the most potent agent in acne treatment. Surprisingly, its mechanism of action in acne is still unknown. Gene expression profiling of skin from 6 patients treated with isotretinoin was performed to gain insights into its mechanism of action. Skin biopsies were obtained from the patients at baseline and at one-week isotretinoin treatment. Gene array expression profiling was conducted using Affymetrix HG-U133A 2.0 arrays in order to examine changes in gene expression as a result of treatment. After treatment, 43 genes were significantly changed: 38 up-regulated and 5 down-regulated. A significant proportion of these genes are involved in pathways that regulate differentiation, tumor suppression, serine proteases, serine protease inhibitors and solute transfer. These studies are the first describing the initial changes in gene expression associated with isotretinoin therapy and are valuable in identifying potential therapeutic targets in acne. Experiment Overall Design: Total 12 chips: 6 baseline/before isotretinoin and 6 after 1-week isotretinoin treatment.

ORGANISM(S): Homo sapiens

SUBMITTER: Diane Thiboutot 

PROVIDER: E-GEOD-10433 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Neutrophil gelatinase-associated lipocalin mediates 13-cis retinoic acid-induced apoptosis of human sebaceous gland cells.

Nelson Amanda M AM   Zhao Wei W   Gilliland Kathryn L KL   Zaenglein Andrea L AL   Liu Wenlei W   Thiboutot Diane M DM  

The Journal of clinical investigation 20080401 4


13-cis retinoic acid (13-cis RA; also known as isotretinoin) is the most potent agent available for treatment of acne. It is known that the drug induces apoptosis in cells cultured from human sebaceous glands, but its mechanism of action has not been determined. In this study, skin biopsies were taken from 7 patients with acne prior to and at 1 week of treatment with 13-cis RA. TUNEL staining confirmed that 13-cis RA induced apoptosis in sebaceous glands. Transcriptional profiling of patient ski  ...[more]

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