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Tissue-Specific Transcriptional Profiling of Epigenetic Asymmetry in Porcine Parthenogenetic Fetuses1

ABSTRACT: Using two complementary approaches, analysis of imprinting of candidate genes by pyrosequencing and expression profiling of parthenogenetic fetuses, we carried a comprehensive survey of genomic imprinting in swine. In the case of imprinted genes where transcription of one of the two parental alleles is silenced, uniparental embryos like parthenotes can be used to measure transcript dosage effects. Using Affymetrix Porcine GeneChip microarrays, four tissues of day 30 fetuses were profiled: brain, fibroblast, liver, and placenta. Keywords: Transcriptional profiling of epigenetic asymmetry in porcine day 30 parthenogenetic and biparental fetal tissues 24 samples total of Day 30 occidental porcine fetuses: 2 treatments (parthenote, control); four tissues (brain, fibroblast, liver, placenta); technical replicates (3 fibroblast only)

ORGANISM(S): Sus scrofa

SUBMITTER: Nicholas Hardison 

PROVIDER: E-GEOD-10443 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Characterization of conserved and nonconserved imprinted genes in swine.

Bischoff Steve R SR   Tsai Shengdar S   Hardison Nicholas N   Motsinger-Reif Alison A AA   Freking Brad A BA   Nonneman Dan D   Rohrer Gary G   Piedrahita Jorge A JA  

Biology of reproduction 20090701 5

To increase our understanding of imprinted genes in swine, we carried out a comprehensive analysis of this gene family using two complementary approaches: expression and phenotypic profiling of parthenogenetic fetuses, and analysis of imprinting by pyrosequencing. The parthenote placenta and fetus were smaller than those of controls but had no obvious morphological differences at Day 28 of gestation. By Day 30, however, the parthenote placentas had decreased chorioallantoic folding, decreased ch  ...[more]

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