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Transcription profiling of estradiol-treated human breast cancer cells expressing mutant estrogen receptors

ABSTRACT: MDA-MB-231 Breast Cancer Cells expressing either wild-type estrogen receptor or the mutant estrogen recepor L540Q were treated with estradiol for 1 or 2 hours

ORGANISM(S): Homo sapiens

SUBMITTER: Joshua David Stender

PROVIDER: E-GEOD-1045 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Selective recognition of distinct classes of coactivators by a ligand-inducible activation domain.

Acevedo Mari Luz ML Lee Kathleen C KC Stender Joshua D JD Katzenellenbogen Benita S BS Kraus W Lee WL

Molecular cell 20040301 5

How nuclear receptors (NRs) coordinate the sequential, ligand-dependent recruitment of multiple coactivator complexes (e.g., SRC complexes and Mediator) that share similar receptor binding determinants is unclear. We show that although the receptor binding subunits of these complexes (i.e., SRCs and Med220, respectively) share overlapping binding sites on estrogen receptor alpha (ERalpha), information contained in the receptor-coactivator interface allows the receptor to distinguish between them ...[more]

PMID: 15023342

Similar Datasets

Estradiol Treated Breast Cancer Cells Expressing Mutant Estrogen Receptors

Project description:MDA-MB-231 Breast Cancer Cells expressing either wild-type estrogen receptor or the mutant estrogen recepor L540Q were treated with estradiol for 1 or 2 hours Keywords: time-course

2004-03-24 | GSE1045 | GEO

Transcription profiling of human breast cancer cells expressing ERalpha and treated with 17beta-estradiol

Project description:Gene expression changes caused by estrogen treatment of breast cancer cells that re-express ERalpha was investigated by infecting ER-negative MDA-MB-231 breast cancer cells for 24 h with recombinant adenovirus encoding full-length human ERalpha (Ad-ERalpha) or control vector (Ad-LacZ), and treating them with 0Â·01% ethanol (vehicle control) or 10-8 M 17beta-estradiol (E2). After 48 h of treatment, total RNA was isolated and used for transcript profiling on Affymetrix GeneChips. Three independent biological replicates of this experiment were carried out.

2007-08-25 | E-GEOD-2251 | biostudies-arrayexpress

Effects of retinoids on estrogen-receptor-positive and -negative breast carcinoma cells: mRNA profiling

2010-05-21 | GSE18390 | GEO

Effects of retinoids on estrogen-receptor-positive and -negative breast carcinoma cells: mRNA profiling

Project description:The overall study explores differential sensitivity of estrogen-receptor-positive and -negative breast carcinoma cells to retinoids via gene expression and microRNA profiling in MCF7 and MDA-MB-231 cells. This Series reports results of transcriptional profiling of breast carcinoma cell lines comparing the effects of retinoic acid treatment (6 and 48 hours) on estrogen-receptor-positive (MCF7) and estrogen-receptor-negative (MDA-MB-231) cells. mRNA profiling: Retinoic-acid-treated (1microM) vs vehicle-treated cells, two time points (6 and 48h), two cell lines (MCF7 and MDA-MB-231). Two biological replicates for each condition, balanced dye design.

2010-05-21 | E-GEOD-18390 | biostudies-arrayexpress

MicroRNA profiling shows different response to retinoids in estrogen-receptor positive and negative cells

Project description:This Series reports results of miRNA profiling of estrogen-receptor-positive (MCF7) and estrogen-receptor-negative (MDA-MB-231) cells. Retinoic Acid (RA) induces mir-21 in MCF-7 but not in MDA-MB-231 cells. MCF-7 and MDA-MB-231 cells were treated (or not) with retinoic acid (RA) and grown for either 6 hours or 48 hours.

2010-05-21 | GSE18628 | GEO

MicroRNA profiling shows different response to retinoids in estrogen-receptor positive and negative cells

2010-05-21 | E-GEOD-18628 | biostudies-arrayexpress

A novel oncogenic enhancer of estrogen receptor positive breast cancer

Project description:To understand the regulatory landscape of oncogene c-Myc in breast cancer, two epigenetic hallmarks of open chromatin, consisting of H3K4me1 and H3K27ac, were investigated by ChIP-seq in distinct types of human breast cancer cells (estrogen receptor positive MCF-7 and triple-negative MDA-MB-231), respectively. By visualizing the ChIP-seq at c-Myc locus, we noticed that the H3K4me1 and H3K27ac signals were enriched remarkably upstream ~65 kb of c-Myc in MCF-7 cells but not in MDA-MB-231 cells. The results indicated that c-Myc may be regulted by an enhancer located upstream ~65 kb.

2022-10-04 | GSE180080 | GEO

Research resource: global identification of estrogen receptor ? target genes in triple negative breast cancer cells

Project description:The goal of this work was to identify all estrogen receptor beta target genes using RNA sequencing in MDA-MB-468 triple negative breast cancer cells engineered with inducible expression of full length estrogen receptor beta. MDA-MB-468 breast cancer cells with inducible ERb expression (MDA-468-ERb cells) were treated in triplicate with vehicle (control, no ERb) or doxycycline (plus ERb) for 48 hr prior to treatment with 0.1% DMSO vehicle or 10 nM 17b-estradiol for 4 hr.

2015-08-12 | E-GEOD-71960 | biostudies-arrayexpress

17β-estradiol regulates the release of exosomes via miR-1495p and hnRNPA2/B1 in ER+ breast cancer cells

Project description:We analyzed RNA from 2 breast cancer cell lines (MDA-MB-231 (MM231) and MCF7) and exosomes derived from them We used Affymetrix miRNA-3 array to determine the miRNA profile to find out whether miRs content in exosomes and expression levels were different after 17β-estradiol .

2020-03-01 | GSE127787 | GEO

Time-course effect of estradiol and ERa17p on Early Gene expression in human breast cancer cell lines

Project description:This SuperSeries is composed of the following subset Series: GSE39718: Time-course effect of estradiol and ERa17p on Early Gene expression in MDA-MB-231 cells GSE39719: Time-course effect of estradiol and ERa17p on Early Gene expression in SKBR3 cells GSE39720: Time-course effect of estradiol and ERa17p on Early Gene expression in T47D cells Refer to individual Series

2012-07-30 | E-GEOD-39721 | biostudies-arrayexpress

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