Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Arabidopsis thaliana rosettes during chilling

ABSTRACT: To investigate the response of Arabidopsis thaliana plants to non-freezing, cool temperatures, we subjected four week old plants to various chilling temperatures at defined times during the diurnal cycle to control for diurnal effects on transcription. From the same plants, metabolites and enzyme activities were measured as well. Interestingly a gradual change could be observed over a wide range of temperatures. Some of which could be attributed to the CBF program. Experiment Overall Design: Arabidopsis thaliana rosettes from 4 week old plants at a time point four hours into the light-period were transfered to various "chilling" temperatures (20 [control], 17, 14, 12, 10 and 8Â°C] and harvested after 6 or 78 hours (both 10 hours into the light period). Experiment Overall Design: 6 continuous treatments X 2 timepoints X 2 replicates

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Björn Usadel

PROVIDER: E-GEOD-10522 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Multilevel genomic analysis of the response of transcripts, enzyme activities and metabolites in Arabidopsis rosettes to a progressive decrease of temperature in the non-freezing range.

Usadel Björn B Bläsing Oliver E OE Gibon Yves Y Poree Fabien F Höhne Melanie M Günter Manuela M Trethewey Richard R Kamlage Beate B Poorter Hendrik H Stitt Mark M

Plant, cell & environment 20071210 4

This paper characterizes the transcriptional and metabolic response of a chilling-tolerant species to an increasingly large decrease of the temperature. Arabidopsis Col-0 was grown at 20 degrees C and transferred to 17, 14, 12, 10 or 8 degrees C for 6 and 78 h, before harvesting the rosette and profiling >22 000 transcripts, >20 enzyme activities and >80 metabolites. Most parameters showed a qualitatively similar response across the entire temperature range, with the amplitude increasing as the ...[more]

PMID: 18088337

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Dataset Information

Transcription profiling of Arabidopsis thaliana rosettes during chilling

Publications

Multilevel genomic analysis of the response of transcripts, enzyme activities and metabolites in Arabidopsis rosettes to a progressive decrease of temperature in the non-freezing range.

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