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Polycomb CBX7 represses expression of tumor suppressor genes

ABSTRACT: Polycomb CBX7 was stably expressed in embryonal carcinoma cells in order to identify target genes. The study was specifically geared toward unveiling gene targets that are susceptible to DNA hypermethylation in adult cancer. A number of CBX7 target tumor suppressor genes were thereby identified and used in further studies to characterize the mechanism surrounding CBX7 mediated gene suppression. Keywords: CBX7 target gene identification A cDNA encoding the ORF of polycomb CBX7 under the control of EF1alpha promoter was transfected into the Tera-2 embryonal carcinoma cell line. Empty vector was used to transfect control cells. The cells were subject to stable selection using puromycin and a pooled population of CBX7 overexpressing cells was established. Gene expression analysis was performed by comparing the gene expression profile of CBX7 cells to empty vector control cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Leander Van Neste 

PROVIDER: E-GEOD-10541 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Polycomb CBX7 promotes initiation of heritable repression of genes frequently silenced with cancer-specific DNA hypermethylation.

Mohammad Helai P HP   Cai Yi Y   McGarvey Kelly M KM   Easwaran Hariharan H   Van Neste Leander L   Ohm Joyce E JE   O'Hagan Heather M HM   Baylin Stephen B SB  

Cancer research 20090714 15

Epigenetic silencing of genes in association with aberrant promoter DNA hypermethylation has emerged as a significant mechanism in the development of human cancers. Such genes are also often targets of the polycomb group repressive complexes in embryonic cells. The polycomb repressive complex 2 (PRC2) has been best studied in this regard. We now examine a link between PRC1 and cancer-specific gene silencing. Here, we show a novel and direct association between a constituent of the PRC1 complex,  ...[more]

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