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Methotrexate treatment-dynamics of Arabidopsis transcriptome during folate depletion

ABSTRACT: cea06-02_folate - methotrexate treatment - global analysis of the folate metabolism in Arabidopsis - Treatment of cells of Arabidopsis by methotrexate which is an inhibitor of the synthesis of the folate. Consequently, the pool of folates presents a strong reduction. The goal of this project is to look at the variations of expression in the Arabidopsis genome and specifically in some genes implied in the metabolism of the folate. Keywords: time course,treated vs untreated comparison 10 dye-swap - CATMA arrays

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Agnes Yu

PROVIDER: E-GEOD-10675 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A genome-wide and metabolic analysis determined the adaptive response of Arabidopsis cells to folate depletion induced by methotrexate.

Loizeau Karen K De Brouwer Veerle V Gambonnet Bernadette B Yu Agnès A Renou Jean-Pierre JP Van Der Straeten Dominique D Lambert Willy E WE Rébeillé Fabrice F Ravanel Stéphane S

Plant physiology 20081017 4

Control of folate homeostasis is essential to sustain the demand for one-carbon (C1) units that are necessary for major biological functions, including nucleotide synthesis and methylation reactions. In this study, we analyzed the genome-wide and metabolic adaptive response of Arabidopsis (Arabidopsis thaliana) cells to folate depletion induced by the antifolate methotrexate. Drug treatment induced a response typical to xenobiotic stress and important changes in folate content and composition. T ...[more]

PMID: 18931140

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