Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

34 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Overexpression of the transcription factor GAT201 in Cryptococcus neoformans var. grubii (serotype A, H99)

ABSTRACT: Comparison of transcriptional profiles of WT Cryptococcus neoformans (H99) and strain CM126 (pRPL2b-GAT201) which overexpresses the transcription factor GAT201 using a ribosomal protein promoter Keywords: Genetic modification WT vs. CM126 competitive hybridization. 4 biological replicates including 2 dye flips. Cultures grown at 37 degress Celsius in minimal (YNB) medium. Cultures independently grown and harvested during exponential growth.

ORGANISM(S): Filobasidiella neoformans

SUBMITTER: Changbin Chen

PROVIDER: E-GEOD-10750 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Systematic genetic analysis of virulence in the human fungal pathogen Cryptococcus neoformans.

Liu Oliver W OW Chun Cheryl D CD Chow Eric D ED Chen Changbin C Madhani Hiten D HD Noble Suzanne M SM

Cell 20081001 1

The fungus Cryptococcus neoformans is a leading cause of mortality and morbidity among HIV-infected individuals. We utilized the completed genome sequence and optimized methods for homologous DNA replacement using high-velocity particle bombardment to engineer 1201 gene knockout mutants. We screened this resource in vivo for proliferation in murine lung tissue and in vitro for three well-recognized virulence attributes-polysaccharide capsule formation, melanization, and growth at body temperatur ...[more]

PMID: 18854164

Similar Datasets

Hypoxia response in C. neoformans in H99, CM092 and CM098

Project description:(1) The identification of genes that are transcriptionally regulated in response to exposure to hypoxia (~0.2% oxygen) in H99 (WT) and strains CM092 and CM098. (2) The identification of genes that are differentially-regulated in the strain CM093 compared to H99 (WT) Keywords: hypoxia response, comparative transcriptional hybridization 8 cultures for each strain (4 grown in normoxia and 4 grown in hypoxia) are analyzed on 4 microarrays for each strain (normoxia hybridized against hypoxia). Two out of four microarrays contain dye-swaps.

2010-06-12 | E-GEOD-6226 | biostudies-arrayexpress

Schladebeck_Whi3_Ploidy

Project description:We analyzed and classified Whi3-regulated and ploidy-regulated genes in haploid and diploid strains of the Sigma1278b genetic background under vegetative growth conditions.<br><br>For this purpose, we measured transcriptional profiles of two different haploid MATa and one diploid MATa/a yeast strains of the following genotypes: WHI3 strain (SS_YHUM468=YHUM0468), whi3 strain (SS_ySS137=YHUM1920) and whi3-delta/whi3-delta strain (SS_ySS137dipl=YHUM2152). All three strains were grown in duplicate in YNB medium supplemented with tryptophan and uracil at 30 degrees C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling<br><br>

2013-06-19 | E-MEXP-3610 | biostudies-arrayexpress

Transcription profiling by array of yeast haploid Sigma1278b wildtype strain and whi3 deletion strain

Project description:We determined and classified Whi3-regulated genes in haploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of two different haploid MATa yeast strains of the following genotypes: WHI3 strain (468=YHUM0468) and whi3 strain (1105=YHUM1105).Both starins were grown in duplicate in YNB medium with tryptophan at 30°C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling

2011-07-08 | E-MEXP-2810 | biostudies-arrayexpress

Response of Candida glabrata to low pH

Project description:To examine the role of a glycosylphosphatidylinositol-linked aspartyl protease, CgYps1, in the regulation of pH homeostasis in Candida glabrata, transcriptional profiling analysis was carried out on wild-type and Cgyps1∆ cells grown in YNB medium (pH 5.5) and in YNB medium adjusted to pH 2.0. Genes involved in carbohydrate and amino acid metabolism, protein folding and stress response pathways were found to be differentially regulated in response to acidic environment in both the strains. To examine the role of a glycosylphosphatidylinositol-linked aspartyl protease, CgYps1, in the regulation of pH homeostasis in Candida glabrata, transcriptional profiling analysis was carried out on wild-type and Cgyps1∆ delta cells grown in YNB medium (pH 5.5) and in YNB medium adjusted to pH2.0. Genes involved in carbohydrate and amino acid metabolism, protein folding and stress response pathways were found to be differentially regulated in response to acidic environment in both the strains Agilent one-color experiment,Organism: Yeast ,Agilent-026378 Genotypic designed Custom Candida glabrata 8x15k , Labeling kit: Agilent Quick-Amp labeling

2011-01-10 | E-GEOD-24267 | biostudies-arrayexpress

Transcription profiling by array of yeast haploid Sigma1278b strains to detect Yak1-regulated genes

Project description:We determined and classified Yak1-regulated genes in haploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of different haploid MATa yeast strains of the following genotypes:YAK1 (468=YHUM0468), yak1 (1313=YHUM1313), strain YHUM1313 (yak1) carrying a high copy plasmid with YAK1 (1313Yhc) and strain YHUM1313 (yak1) carrying a high copy plasmid with YAK1 K398R (1313KDhc). All strains were grown in duplicate in YNB medium supplemented with tryptophan at 30 degrees Celsius to an optical density of 1.0 before extraction of total RNA and transcriptional profiling.

2011-08-23 | E-MEXP-2885 | biostudies-arrayexpress

Expression data from diclofenac-treated yeast cells

Project description:Diclofenac is a widely used analgesic drug that can cause serious adverse drug reactions. We used Saccharomyces cerevisiae as model eukaryote to elucidate the molecular mechanisms of diclofenac toxicity and resistance. Although most yeast cells died during initial diclofenac treatment, some survived and started growing again. Microarray analysis of the adapted cells identified three major processes involved in diclofenac detoxification and tolerance. Especially pleiotropic drug resistance genes and genes under control of Rlm1p, a transcription factor in the protein kinase C (PKC) pathway, were upregulated in diclofenac-adapted cells. Genes involved in ribosome biogenesis and rRNA processing were downregulated, as well as zinc-responsive genes. Five independent yeast cultures were incubated with 100 ÂµM diclofenac for 75 hours. Every 24h, cultures were diluted to OD600 ~0.1 in YNB containing 100 ÂµM diclofenac. Five independent control cultures were grown in the absence of diclofenac.

2011-05-17 | E-GEOD-29331 | biostudies-arrayexpress

Overexpression of the transcription factor GAT201 in Cryptococcus neoformans var. grubii (serotype A, H99)

Project description:Comparison of transcriptional profiles of WT Cryptococcus neoformans (H99) and strain CM126 (pRPL2b-GAT201) which overexpresses the transcription factor GAT201 using a ribosomal protein promoter Keywords: Genetic modification

2008-03-07 | GSE10750 | GEO

Lactate sensing drives fungal immune evasion

Project description:Experiment Description RNA sequencing was performed on Candida albicans wild type cells (SC5314) grown to exponential phase on YNB Lactate, YNB Glucose, or YNB Glucose plus Lactate, and compared to exponential Candida albicans crz1 cells grown on YNB Glucose or YNB Glucose plus Lactate. Three independent experiments were performed.

2016-10-03 | E-MTAB-4579 | biostudies-arrayexpress

Evolutionary engineering of Saccharomyces cerevisiae for enhanced tolerance to hydrolysates of lignocellulosic biomass Samples vs Reference

Project description:Adaptive evolution experiment for enhaced tolerance to hydrolysates of lignocellulosic biomass in S. cerevisiae. The samples involves a batch culture in YNB and Hydrolysates. Cells were harvested at mid-exponential phase. Three biological replicas of 8 samples vs reference Samples 1-8 are the first replicate, Samples 9-16 are the second replicate, Samples 17-24 are the third replicate.

2013-04-26 | E-GEOD-44085 | biostudies-arrayexpress

Transcription profiling of yeast transcription factor knockout compendium

Project description:We reprocessed a recently published microarray compendium of 269 regulator knockouts that contains most known TFs and many epigenetic regulators for the yeast \italic{Saccharomyces cerevisiae} (Hu et al 2007). Using sensitive microarray analysis tools, we gained nearly 10 times more differentially expressed genes and recovered 90\% of previously described targets. We validated our analysis using Gene Ontology (GO), DNA-protein interactions, TF binding sites and protein-protein interactions. Our validation underlines the advantages of our sensitive analysis. The original data files for this submission can be found in E-MTAB-109.additional.zip

2010-03-22 | E-MTAB-109 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data