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Overexpression of the transcription factor GAT201 in Cryptococcus neoformans var. grubii (serotype A, H99)

ABSTRACT: Comparison of transcriptional profiles of WT Cryptococcus neoformans (H99) and strain CM126 (pRPL2b-GAT201) which overexpresses the transcription factor GAT201 using a ribosomal protein promoter Keywords: Genetic modification WT vs. CM126 competitive hybridization. 4 biological replicates including 2 dye flips. Cultures grown at 37 degress Celsius in minimal (YNB) medium. Cultures independently grown and harvested during exponential growth.

ORGANISM(S): Filobasidiella neoformans

SUBMITTER: Changbin Chen

PROVIDER: E-GEOD-10750 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematic genetic analysis of virulence in the human fungal pathogen Cryptococcus neoformans.

Liu Oliver W OW Chun Cheryl D CD Chow Eric D ED Chen Changbin C Madhani Hiten D HD Noble Suzanne M SM

Cell 20081001 1

The fungus Cryptococcus neoformans is a leading cause of mortality and morbidity among HIV-infected individuals. We utilized the completed genome sequence and optimized methods for homologous DNA replacement using high-velocity particle bombardment to engineer 1201 gene knockout mutants. We screened this resource in vivo for proliferation in murine lung tissue and in vitro for three well-recognized virulence attributes-polysaccharide capsule formation, melanization, and growth at body temperatur ...[more]

PMID: 18854164

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