Transcription profiling of mouse liver to understand the regulation of hepatic gene expression by dwarfism and caloric restriction

ABSTRACT: Mice were killed between 24 to 26 months of age, tissues removed, rapidly frozen on dry ice, and stored in liquid nitrogen. Total liver RNA was isolated from frozen tissue as described (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted). mRNA levels were measured using the Affymetrix mouse U74Av2 array according to standard protocols. After hybridization, arrays were scanned using a Hewlett-Packard GeneArray Scanner. Image analysis was performed as described (Cao SX, Dhahbi JM, Mote PL, and Spindler SR. Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice. Proc Natl Acad Sci U S A 98: 10630-10635, 2001). A more detailed description of the methods can be found in (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted). This SuperSeries is composed of the following subset Series: GSE1086: Dwarf Ad libitum; GSE1087: Dwarf Calorie Restricted; GSE1088: Normal Ad Libitum; GSE1089: Normal Calorie Restricted Experiment Overall Design: Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Stephen Spindler 

PROVIDER: E-GEOD-1093 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress