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Transcription profiling of human glioblastoma multiforme tumors taken before dendritic cell vaccination, recurrent tumors after vaccination and control GBM tumors

ABSTRACT: Human Glioblastoma Multiforme tumors taken before dendritic cell vaccination, the recurrent tumors taken after vaccination and control GBM tumors from non vaccinated patients. Experiment Overall Design: Six Glioblastoma Multiforme patients underwent surgery. Their brain tumors were removed and analyzed via microarray. The lysate from the tumors were cultured with the patients' dendritic cells and the DCs were injected back into the patients. The patients GBMs returned and they underwent surgery a second time and those tumors were also analyzed via microarray. Tumors from the first and second GBM surgeries of 5 patients who did not receive DC vaccines are included as controls.

ORGANISM(S): Homo sapiens

SUBMITTER: Gretchen Duvall

PROVIDER: E-GEOD-11100 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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T cells enhance stem-like properties and conditional malignancy in gliomas.

Irvin Dwain K DK Jouanneau Emmanuel E Duvall Gretchen G Zhang Xiao-Xue XX Zhai Yuying Y Sarayba Danielle D Seksenyan Akop A Panwar Akanksha A Black Keith L KL Wheeler Christopher J CJ

PloS one 20100607 6

<h4>Background</h4>Small populations of highly tumorigenic stem-like cells (cancer stem cells; CSCs) can exist within, and uniquely regenerate cancers including malignant brain tumors (gliomas). Many aspects of glioma CSCs (GSCs), however, have been characterized in non-physiological settings.<h4>Methods</h4>We found gene expression similarity superiorly defined glioma "stemness", and revealed that GSC similarity increased with lower tumor grade. Using this method, we examined stemness in human ...[more]

PMID: 20539758

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