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Transcription profiling of mouse MC3T3 cells treated with B-glycerol phopsphate/ascorbic acid/bone morphogenetic protein 2 (osteogenic stimuls) vs. controls

ABSTRACT: MC3T3 cells clone 1b were grown in alpha-MEM with 10% fetal calf serum (FCS, Gibco), 1% penicilin/streptomycin (Pen/Strep) and 1% L-Glutamine (L-Glu). For differentiation experiment, cells were seeded on 6 cm dishes (3x105cells/dish in 5 ml medium) and grownt to confluence for 3 days at 37C / 5% CO2. Cells were then stimulated with osteogenic stimulus (10 mM beta-glycerophosphate (GP, Sigma), 50 µM ascorbic acid (ascorbic acid, Wako) and 1 mg/ml bone morphogenetic protein 2 (Novartis)), or with 10mM GP alone for 1 or 3 days. Control cells-day 0, medium. Experiment was done in triplicate. Total RNA was extracted, treated with DNAse, and purified according to the manufacturer’s protocol (RNeasy mini kit, QIAGEN). RNA samples were analyzed on oligonucleotide microarrays Affymetrix GeneChip® Murine Genome U74Av2.

ORGANISM(S): Mus musculus

SUBMITTER: Natasa Zamurovic 

PROVIDER: E-GEOD-1131 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Coordinated activation of notch, Wnt, and transforming growth factor-beta signaling pathways in bone morphogenic protein 2-induced osteogenesis. Notch target gene Hey1 inhibits mineralization and Runx2 transcriptional activity.

Zamurovic Natasa N   Cappellen David D   Rohner Daisy D   Susa Mira M  

The Journal of biological chemistry 20040602 36

To examine early events in osteoblast differentiation, we analyzed the expression of about 9,400 genes in the murine MC3T3 cell line, whose robust differentiation was documented cytochemically and molecularly. The cells were stimulated for 1 and 3 days with the osteogenic stimulus containing bone morphogenic protein 2. Total RNA was extracted and analyzed by Affymetrix GeneChip oligonucleotide arrays. A regulated expression of 394 known genes and 295 expressed sequence tags was detected. The sen  ...[more]

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