Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional analysis of respiratory pathways in the hyperthermophilic crenarchaeon Pyrobaculum aerophilum


ABSTRACT: We compared global transcriptional patterns in Pyrobaculum aerophilum cultures with oxygen, nitrate, arsenate and ferric iron as respiratory electron acceptors to identify genes and regulatory patterns that differentiate these pathways. Keywords: Time course study with different respiratory electron acceptors To focus the microarrays on genes that are specifically affected by changes in terminal electron acceptors we analyzed gene expression in time courses with cultures shifted from aerobic growth to either NO3-, As(V), Fe(III)-citrate, or additional O2. Gene expression and substrate usage were measured at three time points (2.5, 4.5, and 7.5 hours), allowing sufficient time for changes in gene expression, while restricting cultures to a maximum of one or two cell divisions.

ORGANISM(S): Pyrobaculum aerophilum

SUBMITTER: Aaron Cozen 

PROVIDER: E-GEOD-11366 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional map of respiratory versatility in the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.

Cozen Aaron E AE   Weirauch Matthew T MT   Pollard Katherine S KS   Bernick David L DL   Stuart Joshua M JM   Lowe Todd M TM  

Journal of bacteriology 20081201 3


Hyperthermophilic crenarchaea in the genus Pyrobaculum are notable for respiratory versatility, but relatively little is known about the genetics or regulation of crenarchaeal respiratory pathways. We measured global gene expression in Pyrobaculum aerophilum cultured with oxygen, nitrate, arsenate and ferric iron as terminal electron acceptors to identify transcriptional patterns that differentiate these pathways. We also compared genome sequences for four closely related species with diverse re  ...[more]

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