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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of human dermal fibroblasts to determine passage dependent gene expression

ABSTRACT: Human umbilical vein endothelial cells (HUVECs) formed capillary structures when co-cultured with normal human dermal fibroblasts (NHDFs). HUVEC competence and NHDF supportiveness of cord formation were found to be highly cell-passage dependent with the early passage cells forming more angiogenic cord structures. We thus profiled gene expression in NHDFs with different passages to understand the molecular mechanisms underlying the in vitro angiogenesis control. Experiment Overall Design: NHDFs cultured to various passages were assayed for their gene expression using the GeneChip technology.

ORGANISM(S): Homo sapiens

SUBMITTER: Tao Wei

PROVIDER: E-GEOD-11418 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Developing and applying a gene functional association network for anti-angiogenic kinase inhibitor activity assessment in an angiogenesis co-culture model.

Chen Yuefeng Y Wei Tao T Yan Lei L Lawrence Frank F Qian Hui-Rong HR Burkholder Timothy P TP Starling James J JJ Yingling Jonathan M JM Shou Jianyong J

BMC genomics 20080602

<h4>Background</h4>Tumor angiogenesis is a highly regulated process involving intercellular communication as well as the interactions of multiple downstream signal transduction pathways. Disrupting one or even a few angiogenesis pathways is often insufficient to achieve sustained therapeutic benefits due to the complexity of angiogenesis. Targeting multiple angiogenic pathways has been increasingly recognized as a viable strategy. However, translation of the polypharmacology of a given compound ...[more]

PMID: 18518970

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