Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Spneu D39 wildtype grown in GM17 versus GM17 + 0.25 mM ZnSO4

ABSTRACT: This study addresses the response of Streptococcus pneumoniae to zinc. The wild-type strain D39 was grown in GM17 and GM17 with 0.25 mM of ZnSO4. The transcriptome under these conditions was compared by using microarrays. Keywords: transcriptome analysis of D39 wild-type compared with its isogenic glnPA double mutant The wild-type strain D39 was grown in 4 biological replicates in GM17 and in GM17+0.25 mM ZnSO4 to an Optical Density at 600 nm of 0.3. From these cultures samples were prepared for transciptome determination with in-house printed microarrays covering the genomes of several S. pneumoniae species. The GM17 samples (CH1) and GM17+ZnSO4 samples (CH2) were labeled with Cy5 or Cy3. Samples were hybridized to glass-microarray slides. This work is part of a publication that is likely to be released in june/july 2008: 'Opposite effects of Mn2+ and Zn2+ on the PsaR-mediated expression of the virulence genes pcpA, prtA and psaBCA of Streptococcus pneumoniae" by Kloosterman et al.

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Anne Jong 

PROVIDER: E-GEOD-11438 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Opposite effects of Mn2+ and Zn2+ on PsaR-mediated expression of the virulence genes pcpA, prtA, and psaBCA of Streptococcus pneumoniae.

Kloosterman Tomas G TG   Witwicki Robert M RM   van der Kooi-Pol Magdalena M MM   Bijlsma Jetta J E JJ   Kuipers Oscar P OP  

Journal of bacteriology 20080530 15

Homeostasis of Zn(2+) and Mn(2+) is important for the physiology and virulence of the human pathogen Streptococcus pneumoniae. Here, transcriptome analysis was used to determine the response of S. pneumoniae D39 to a high concentration of Zn(2+). Interestingly, virulence genes encoding the choline binding protein PcpA, the extracellular serine protease PrtA, and the Mn(2+) uptake system PsaBC(A) were strongly upregulated in the presence of Zn(2+). Using random mutagenesis, a previously described  ...[more]

Similar Datasets

Transcriptomics Spneu D39 wildtype grown in GM17 versus GM17 + 0.25 mM ZnSO4
2008-05-17 | GSE11438 | GEO
Transcriptomics Wild type vs glnPA double mutant
2010-05-26 | E-GEOD-9850 | biostudies-arrayexpress
Transcriptomics Comparison of the Streptococcus pneumoniae D39 lacR Mutant compared to D39 wild-type
2014-06-20 | E-GEOD-58183 | biostudies-arrayexpress
Transcriptomics N-acetylgalactosamine Mediated Regulation of the aga Operon by AgaR in Streptococcus pneumoniae
2016-11-02 | GSE86008 | GEO
Genomics Streptococcus pneumoniae
| PRJNA106467 | ENA
Transcriptomics Impact of rokA delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose
2012-10-22 | GSE41448 | GEO
Transcriptomics Impact of ccpA delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose
2015-03-06 | GSE66558 | GEO
Transcriptomics Impact of bguR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose
2013-02-20 | GSE43342 | GEO
Transcriptomics Impact of lacR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose
2014-06-20 | GSE58183 | GEO
Transcriptomics Impact of galR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose
2015-10-30 | GSE74500 | GEO