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Transcriptome analysis of Escherichia coli O157:H7 EDL933 during heat shock using microarrays

ABSTRACT: The transcriptome of Escherichia coli K-12 has been widely studied over a variety of conditions for the past decade while such studies involving E. coli O157:H7, its pathogenic cousin, are just now being conducted. To better understand the impact of heat shock on E. coli O157:H7, global transcript levels of strain EDL933 cells shifted from 37°C to 50°C for 15 min were compared to cells left at 37°C using microarrays. Keywords: Stress Response Twelve independent RNA samples from heat shocked cultures were paired with twelve independent RNA samples from control cultures for hybridization to twelve two-color microarrays. For six arrays, the control RNA sample was labeled with Cy3 dye and the experimental RNA sample was labeled with Cy5 dye, the dyes were reversed for the other six arrays to overcome dye bias.

ORGANISM(S): Escherichia coli

SUBMITTER: Chris Minion

PROVIDER: E-GEOD-11463 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis of Escherichia coli O157:H7 EDL933 during heat shock.

Carruthers Michael D MD Minion Chris C

FEMS microbiology letters 20090601 1

The response to elevated temperature [heat shock (HS)] is highly conserved. The transcriptome of Escherichia coli K-12 has been studied under a variety of conditions while such studies involving E. coli O157:H7 are only now being conducted. To better understand the impact of HS on E. coli O157:H7, global transcript levels of strain EDL933 cells shifted from 37 to 50 degrees C for 15 min were compared with cells held at 37 degrees C by microarray. Using a mixed model analysis, 193 genes were foun ...[more]

PMID: 19473256

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