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Transcription profiling of male and female human hypothalamus tissue

ABSTRACT: Sex Specific Transciption in Human Hypothalamus between 7 male biological samples (2 technical replicates of each) and 5 female biological samples (2 technical replicates of 4 of these). Post-mordem human hypothalamus total RNA samples were obtained from Ambion (Austin, TX). All total RNA samples has an agilent ratio greater than 1 indicating there had been no degredation. The causes of death varied as well as the age at death, but averaged aproximately 70 years of age. The total RNA samples were labeled for hybridization onto the microarray according to affymetrix protocols listed at: http://keck.med.yale.edu/affymetrix/protocols

ORGANISM(S): Homo sapiens

SUBMITTER: Joel Rozowsky

PROVIDER: E-GEOD-1147 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Major molecular differences between mammalian sexes are involved in drug metabolism and renal function.

Rinn John L JL Rozowsky Joel S JS Laurenzi Ian J IJ Petersen Petur H PH Zou Kaiyong K Zhong Weimin W Gerstein Mark M Snyder Michael M

Developmental cell 20040601 6

Many anatomical differences exist between males and females; these are manifested on a molecular level by different hormonal environments. Although several molecular differences in adult tissues have been identified, a comprehensive investigation of the gene expression differences between males and females has not been performed. We surveyed the expression patterns of 13,977 mouse genes in male and female hypothalamus, kidney, liver, and reproductive tissues. Extensive differential gene expressi ...[more]

PMID: 15177028

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Sex Specific Transciption in Human Hypothalamus

Project description:Sex Specific Transciption in Human Hypothalamus between 7 male biological samples (2 technical replicates of each) and 5 female biological samples (2 technical replicates of 4 of these). Keywords = human hypothalamus, sex-specific transcription Keywords: other

2004-03-28 | GSE1147 | GEO

Transcription profiling of mouse hypothalamus, liver, kidney, ovary, and testes from male and female mice to assess sex specific differences in transcription

Project description:Sex Specific Transciption in Mouse Hypothalamus, Liver, Kidney, Ovary and Testis. For each somatic tissue there are 3 biological samples from different pools comprised of 10 animals for each sex each with a technical replicate. For each of the reproductive tissues there are 3 biological samples from different pools comprised of 10 animals with a technical replicate for each.

2007-09-29 | E-GEOD-1148 | biostudies-arrayexpress

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Project description:The hypothalamus is a functionally and cellularly complex tissue controlling many developmental processes, including puberty. While key hormonal aspects of puberty regulation in the hypothalamus are well established, understanding the genes, cell-types, and epigenetic mechanisms underlying and regulating puberty is limited. Here, we performed 3’-UTR-seq on the hypothalamus from both sexes of C57BL/6J mice at 5 ages spanning pubertal transition (postnatal days 12, 22, 27, 32, 37) (4-5 replicates per sex at each age) to examine genome-wide age- and sex-biased trends in gene expression in a cell-type aware manner. Sample collection, RNA extraction, and sequencing was completed using the same protocols and on the same mice as PMID: 3622112 (E-MTAB-9459). QuantSeq 3’mRNA-seq libraries were constructed from total RNA using an automated method with Agilent NGS Workstation. The resulting single-end libraries were sequenced at SickKids TCAG core on the Illumina v4 flow cell with SR50 bp cycles extended to 68 bp. A customized pipeline was developed and used for the analysis of reads obtained (PMID: 36221127 for details). Processed reads were mapped to mouse genome (mm10).

2023-04-01 | E-MTAB-12340 | biostudies-arrayexpress

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Project description:Sex Specific Transciption in Human Hypothalamus

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Hypothalamus-specific NSCs derived from human brain organoids ameliorate age-associated dysfunction upon transplantation into aged mouse hypothalamus [mouse]

Project description:The hypothalamus is the brain region that regulates systemic body metabolism and multiple functions in other brain regions. In adult mice, the hypothalamus harbors neural stem/precursor cell (NSC)-like cells. Along with the dysregulation of body metabolism and physiology that occurs during aging, the NSC population in the hypothalamus declines with age. Here, we introduce a novel protocol that yields scalable and storable hypothalamus-specific NSCs (htNSCs) from hypothalamus-like organoids derived from human pluripotent stem cells (hPSCs). Implanting htNSCs into the medio-basal hypothalami of aged mice conspicuously ameliorated age-related declines in metabolic fitness, physical capacity, and cognitive function and produced corresponding histologic changes in various body tissues. Single transcriptome and immunohistochemical analyses of the grafted hypothalamic tissues showed that the anti-aging effects were attained by correcting glial NF-κB, TNFα, and NLRP3 inflammasome pathways. Collectively, our findings support the potential of anti- or healthy aging therapies that target htNSCs and hypothalamic inflammation.

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Hypothalamus-specific NSCs derived from human brain organoids ameliorate age-associated dysfunction upon transplantation into aged mouse hypothalamus [human bulk RNAseq]

2022-10-11 | GSE214877 | GEO

Project description:This study aims to investigate the relationship between the hypothalamus and age-related DNA methylation in mouse model. The objective of this sequencing work is to compare the hypothalamus (HT) with two other components of the limbic system in mice, the hippocampus (HC) and the olfactory bulb (OB), to understand how DNA methylation levels in these regions are similarly versus differently altered as animals evolve from young age to middle age.

2024-10-08 | GSE276875 | GEO

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